2k8c: Difference between revisions
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==Solution structure of PLAA family ubiquitin binding domain (PFUC) trans isomer in complex with ubiquitin== | ==Solution structure of PLAA family ubiquitin binding domain (PFUC) trans isomer in complex with ubiquitin== | ||
<StructureSection load='2k8c' size='340' side='right' caption='[[2k8c]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | <StructureSection load='2k8c' size='340' side='right' caption='[[2k8c]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2k89|2k89]], [[2k8a|2k8a]], [[2k8b|2k8b]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2k89|2k89]], [[2k8a|2k8a]], [[2k8b|2k8b]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPS27A, UBA80, UBCEP1, UBA52, UBCEP2, UBB, UBC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PLAA, PLAP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPS27A, UBA80, UBCEP1, UBA52, UBCEP2, UBB, UBC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PLAA, PLAP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k8c OCA], [http://pdbe.org/2k8c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k8c RCSB], [http://www.ebi.ac.uk/pdbsum/2k8c PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k8c OCA], [http://pdbe.org/2k8c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k8c RCSB], [http://www.ebi.ac.uk/pdbsum/2k8c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k8c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/2k8c_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/2k8c_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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[[Category: Lin, D H]] | [[Category: Lin, D H]] | ||
[[Category: Zhou, C J]] | [[Category: Zhou, C J]] | ||
[[Category: Cytoplasm]] | |||
[[Category: Nucleus]] | [[Category: Nucleus]] | ||
[[Category: Phosphoprotein]] | [[Category: Phosphoprotein]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
[[Category: Ubiquitin in complex with pfuc trans isomer]] | [[Category: Ubiquitin in complex with pfuc trans isomer]] | ||
[[Category: Ubl conjugation]] | |||
[[Category: Wd repeat]] | [[Category: Wd repeat]] | ||
Revision as of 13:41, 18 July 2018
Solution structure of PLAA family ubiquitin binding domain (PFUC) trans isomer in complex with ubiquitinSolution structure of PLAA family ubiquitin binding domain (PFUC) trans isomer in complex with ubiquitin
Structural highlights
Function[PLAP_HUMAN] Involved in the maintenance of ubiquitin levels (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedUbiquitin (Ub) is an essential modifier conserved in all eukaryotes from yeast to human. Phospholipase A(2)-activating protein (PLAA), a mammalian homolog of yeast DOA1/UFD3, has been proposed to be able to bind with Ub, which plays important roles in endoplasmic reticulum-associated degradation, vesicle formation, and DNA damage response. We have identified a core domain from the PLAA family ubiquitin-binding region of human PLAA (residues 386-465, namely PFUC) that can bind Ub and elucidated its solution structure and Ub-binding mode by NMR approaches. The PFUC domain possesses equal population of two conformers in solution by cis/trans-isomerization, whereas the two isomers exhibit almost equivalent Ub binding abilities. This domain structure takes a novel fold consisting of four beta-strands and two alpha-helices, and the Ub-binding site on PFUC locates in the surface of alpha2-helix, which is to some extent analogous to those of UBA, CUE, and UIM domains. This study provides structural basis and biochemical information for Ub recognition of the novel PFU domain from a PLAA family protein that may connect ubiquitination and degradation in endoplasmic reticulum-associated degradation. Structural basis for ubiquitin recognition by a novel domain from human phospholipase A2-activating protein.,Fu QS, Zhou CJ, Gao HC, Jiang YJ, Zhou ZR, Hong J, Yao WM, Song AX, Lin DH, Hu HY J Biol Chem. 2009 Jul 10;284(28):19043-52. Epub 2009 May 7. PMID:19423704[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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