2o5o: Difference between revisions
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|PDB= 2o5o |SIZE=350|CAPTION= <scene name='initialview01'>2o5o</scene>, resolution 1.60Å | |PDB= 2o5o |SIZE=350|CAPTION= <scene name='initialview01'>2o5o</scene>, resolution 1.60Å | ||
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+X+157'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+X+158'>AC2</scene>, <scene name='pdbsite=AC3:No2+Binding+Site+For+Residue+X+159'>AC3</scene> and <scene name='pdbsite=AC4:Mnr+Binding+Site+For+Residue+X+154'>AC4</scene> | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+X+157'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+X+158'>AC2</scene>, <scene name='pdbsite=AC3:No2+Binding+Site+For+Residue+X+159'>AC3</scene> and <scene name='pdbsite=AC4:Mnr+Binding+Site+For+Residue+X+154'>AC4</scene> | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=MNR:PROTOPORPHYRIN+IX+CONTAINING+MN'>MNR</scene>, <scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1mmb|1MMB]], [[2058|2058]], [[205b|205B]], [[2o5l|2O5L]], [[2o5m|2O5M]], [[205q|205Q]], [[205s|205S]], [[2o5t|2O5T]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5o OCA], [http://www.ebi.ac.uk/pdbsum/2o5o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o5o RCSB]</span> | |||
}} | }} | ||
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[[Category: West, A H.]] | [[Category: West, A H.]] | ||
[[Category: Zahran, Z N.]] | [[Category: Zahran, Z N.]] | ||
[[Category: horse heart]] | [[Category: horse heart]] | ||
[[Category: manganese myoglobin]] | [[Category: manganese myoglobin]] | ||
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[[Category: oxygen storage/transport complex]] | [[Category: oxygen storage/transport complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:13:02 2008'' | ||
Revision as of 04:13, 31 March 2008
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| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , and | ||||||
| Ligands: | , , | ||||||
| Related: | 1MMB, 2058, 205B, 2O5L, 2O5M, 205Q, 205S, 2O5T
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Manganese horse heart myoglobin, nitrite modified
OverviewOverview
Nitrite is now recognized as a storage pool of bioactive nitric oxide (NO). Hemoglobin (Hb) and myoglobin (Mb) convert, under certain conditions, nitrite to NO. This newly discovered nitrite reductase activity of Hb and Mb provides an attractive alternative to mammalian NO synthesis from the NO synthase pathway that requires dioxygen. We recently reported the X-ray crystal structure of the nitrite adduct of ferric horse heart Mb, and showed that the nitrite ligand binds in an unprecedented O-binding (nitrito) mode to the d(5) ferric center in Mb(III)(ONO) [D.M. Copeland, A. Soares, A.H. West, G.B. Richter-Addo, J. Inorg. Biochem. 100 (2006) 1413-1425]. We also showed that the distal pocket in Mb allows for different conformations of the NO ligand (120 degrees and 144 degrees ) in Mb(II)NO depending on the mode of preparation of the compound. In this article, we report the crystal structures of the nitrite and NO adducts of manganese-substituted hh Mb (a d(4) system) and of the nitrite adduct of cobalt-substituted hh Mb (a d(6) system). We show that the distal His64 residue directs the nitrite ligand towards the rare nitrito O-binding mode in Mn(III)Mb and Co(III)Mb. We also report that the distal pocket residues allow a stabilization of an unprecendented bent MnNO moiety in Mn(II)MbNO. These crystal structural data, when combined with the data for the aquo, methanol, and azide MnMb derivatives, provide information on the role of distal pocket residues in the observed binding modes of nitrite and NO ligands to wild-type and metal-substituted Mb.
About this StructureAbout this Structure
2O5O is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of manganese- and cobalt-substituted myoglobin in complex with NO and nitrite reveal unusual ligand conformations., Zahran ZN, Chooback L, Copeland DM, West AH, Richter-Addo GB, J Inorg Biochem. 2008 Feb;102(2):216-33. Epub 2007 Aug 25. PMID:17905436
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