2npk: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 2npk |SIZE=350|CAPTION= <scene name='initialview01'>2npk</scene>, resolution 2.00Å | |PDB= 2npk |SIZE=350|CAPTION= <scene name='initialview01'>2npk</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1xqf|1XQF]], [[1xqe|1XQE]], [[1u7g|1U7G]], [[2nmr|2NMR]], [[2nop|2NOP]], [[2now|2NOW]], [[2npc|2NPC]], [[2npd|2NPD]], [[2npe|2NPE]], [[2npg|2NPG]], [[2npj|2NPJ]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npk OCA], [http://www.ebi.ac.uk/pdbsum/2npk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2npk RCSB]</span> | |||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Lupo, D.]] | [[Category: Lupo, D.]] | ||
[[Category: Winkler, F K.]] | [[Category: Winkler, F K.]] | ||
[[Category: ammonia transport]] | [[Category: ammonia transport]] | ||
[[Category: amtb]] | [[Category: amtb]] | ||
| Line 33: | Line 32: | ||
[[Category: membrane protein]] | [[Category: membrane protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:06:28 2008'' | ||
Revision as of 04:06, 31 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | amtB (Escherichia coli) | ||||||
| Related: | 1XQF, 1XQE, 1U7G, 2NMR, 2NOP, 2NOW, 2NPC, 2NPD, 2NPE, 2NPG, 2NPJ
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
An unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductance
OverviewOverview
Amt proteins constitute a class of ubiquitous integral membrane proteins that mediate movement of ammonium across cell membranes. They are homotrimers, in which each subunit contains a narrow pore through which substrate transport occurs. Two conserved histidine residues in the pore have been proposed to be necessary for ammonia conductance. By analyzing 14 engineered polar and non-polar variants of these histidines, in Escherichia coli AmtB, we show that both histidines are absolutely required for optimum substrate conductance. Crystal structures of variants confirm that substitution of the histidine residues does not affect AmtB structure. In a subgroup of Amt proteins, found only in fungi, one of the histidines is replaced by glutamate. The equivalent substitution in E. coli AmtB is partially active, and the structure of this variant suggests that the glutamate side chain can make similar interactions to those made by histidine.
About this StructureAbout this Structure
2NPK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
An unusual twin-his arrangement in the pore of ammonia channels is essential for substrate conductance., Javelle A, Lupo D, Zheng L, Li XD, Winkler FK, Merrick M, J Biol Chem. 2006 Dec 22;281(51):39492-8. Epub 2006 Oct 12. PMID:17040913
Page seeded by OCA on Mon Mar 31 04:06:28 2008