2h6b: Difference between revisions
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==Crystal structure of oxidized CprK in complex with o-chlorophenolacetic acid== | ==Crystal structure of oxidized CprK in complex with o-chlorophenolacetic acid== | ||
<StructureSection load='2h6b' size='340' side='right' caption='[[2h6b]], [[Resolution|resolution]] 2.20Å' scene=''> | <StructureSection load='2h6b' size='340' side='right' caption='[[2h6b]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
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<table><tr><td colspan='2'>[[2h6b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Desha Desha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H6B FirstGlance]. <br> | <table><tr><td colspan='2'>[[2h6b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Desha Desha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H6B FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3C4:(3-CHLORO-4-HYDROXYPHENYL)ACETIC+ACID'>3C4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3C4:(3-CHLORO-4-HYDROXYPHENYL)ACETIC+ACID'>3C4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h6b OCA], [http://pdbe.org/2h6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h6b RCSB], [http://www.ebi.ac.uk/pdbsum/2h6b PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h6b OCA], [http://pdbe.org/2h6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h6b RCSB], [http://www.ebi.ac.uk/pdbsum/2h6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2h6b ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h6/2h6b_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h6/2h6b_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h6b ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
Revision as of 09:41, 27 June 2018
Crystal structure of oxidized CprK in complex with o-chlorophenolacetic acidCrystal structure of oxidized CprK in complex with o-chlorophenolacetic acid
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedHalorespiration is a bacterial respiratory process in which haloorganic compounds act as terminal electron acceptors. This process is controlled at transcriptional level by CprK, a member of the ubiquitous CRP-FNR family. Here we present the crystal structures of oxidized CprK in presence of the ligand ortho-chlorophenolacetic acid and of reduced CprK in absence of this ligand. These structures reveal that highly specific binding of chlorinated, rather than the corresponding non-chlorinated, phenolic compounds in the NH(2)-terminal beta-barrels causes reorientation of these domains with respect to the central alpha-helix at the dimer interface. Unexpectedly, the COOH-terminal DNA-binding domains dimerize in the non-DNA binding state. We postulate the ligand-induced conformational change allows formation of interdomain contacts that disrupt the DNA domain dimer interface and leads to repositioning of the helix-turn-helix motifs. These structures provide a structural framework for further studies on transcriptional control by CRP-FNR homologs in general and of halorespiration regulation by CprK in particular. CprK crystal structures reveal mechanism for transcriptional control of halorespiration.,Joyce MG, Levy C, Gabor K, Pop SM, Biehl BD, Doukov TI, Ryter JM, Mazon H, Smidt H, van den Heuvel RH, Ragsdale SW, van der Oost J, Leys D J Biol Chem. 2006 Sep 22;281(38):28318-25. Epub 2006 Jun 27. PMID:16803881[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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