6c66: Difference between revisions

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-'''Unreleased structure'''
-The entry 6c66 is ON HOLD  until Paper Publication
+==CRISPR RNA-guided surveillance complex, pre-nicking==
+<StructureSection load='6c66' size='340' side='right' caption='[[6c66]], [[Resolution|resolution]] 3.66&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6c66]] is a 15 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C66 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C66 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c66 OCA], [http://pdbe.org/6c66 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c66 RCSB], [http://www.ebi.ac.uk/pdbsum/6c66 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c66 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Type I CRISPR-Cas system features a sequential target-searching and degradation process on double-stranded DNA, by the RNA-guided Cascade complex and the nuclease-helicase fusion enzyme Cas3, respectively. Here we present a 3.7 A resolution cryo-EM structure of the Type I-E Cascade/R-loop/Cas3 complex, poised to initiate DNA degradation. Cas3 distinguishes Cascade conformations and only captures the R-loop forming Cascade, to avoid cleaving partially complementary targets. Its nuclease domain recruits the non-target strand (NTS) DNA at a bulged region for the nicking of single-stranded DNA. An additional 4.7 A resolution cryo-EM structure captures the post-nicking state, in which the severed NTS retracts to the helicase entrance, to be threaded for ATP-dependent processive degradation. These snapshots form the basis for understanding RNA-guided DNA degradation in Type I-E CRISPR-Cas systems.
-Authors:
+Structure basis for RNA-guided DNA degradation by Cascade and Cas3.,Xiao Y, Luo M, Dolan AE, Liao M, Ke A Science. 2018 Jun 7. pii: science.aat0839. doi: 10.1126/science.aat0839. PMID:29880725<ref>PMID:29880725</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6c66" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Ke, A]]
+[[Category: Liao, M]]
+[[Category: Luo, M]]
+[[Category: Xiao, Y]]
+[[Category: Cas3]]
+[[Category: Cascade]]
+[[Category: Crispr-ca]]
+[[Category: Dna binding protein]]
+[[Category: Dna binding protein-dna-rna complex]]