2j5h: Difference between revisions
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|PDB= 2j5h |SIZE=350|CAPTION= <scene name='initialview01'>2j5h</scene> | |PDB= 2j5h |SIZE=350|CAPTION= <scene name='initialview01'>2j5h</scene> | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j5h OCA], [http://www.ebi.ac.uk/pdbsum/2j5h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j5h RCSB]</span> | |||
}} | }} | ||
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[[Category: Ruvo, M.]] | [[Category: Ruvo, M.]] | ||
[[Category: Saporito, A.]] | [[Category: Saporito, A.]] | ||
[[Category: cripto]] | [[Category: cripto]] | ||
[[Category: cysteine-rich domain]] | [[Category: cysteine-rich domain]] | ||
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[[Category: tumour progression]] | [[Category: tumour progression]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:53:15 2008'' | ||
Revision as of 03:53, 31 March 2008
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR ANALYSIS OF MOUSE CRIPTO CFC DOMAIN
OverviewOverview
We report here for the first time the solution structures at pH 3 and pH 6 of the synthetic CFC domain of mouse Cripto and of the point mutated variant W107A that is unable to bind to the Alk4 Cripto receptor. NMR data confirm that the CFC domain has a C1-C4, C2-C6, C3-C5 disulfide pattern and show that structures are rather flexible and globally extended, with three noncanonical antiparallel strands. His104 and Trp107 side chains protrude from a protein edge and are strongly exposed to solvent, supporting previous evidence of direct involvement in receptor binding. On the opposite molecule side, several nonpolar residues are gathered, forming a large hydrophobic patch that supposedly acts as interface with the cell membrane or the adjacent EGF-like domain. A second hydrophilic patch surrounding His104 and Trp107 is present only in the wild type variant, suggesting a possible involvement in modulating Alk4 recognition.
About this StructureAbout this Structure
2J5H is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of mouse Cripto CFC domain and its inactive variant Trp107Ala., Calvanese L, Saporito A, Marasco D, D'Auria G, Minchiotti G, Pedone C, Paolillo L, Falcigno L, Ruvo M, J Med Chem. 2006 Nov 30;49(24):7054-62. PMID:17125258
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