2iz9: Difference between revisions
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|PDB= 2iz9 |SIZE=350|CAPTION= <scene name='initialview01'>2iz9</scene>, resolution 2.85Å | |PDB= 2iz9 |SIZE=350|CAPTION= <scene name='initialview01'>2iz9</scene>, resolution 2.85Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=ONE:1-(BETA-D-RIBOFURANOSYL)-PYRIDIN-4-ONE-5'-PHOSPHATE'>ONE</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iz9 OCA], [http://www.ebi.ac.uk/pdbsum/2iz9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iz9 RCSB]</span> | |||
}} | }} | ||
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[[Category: virus]] | [[Category: virus]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:50:56 2008'' | ||
Revision as of 03:50, 31 March 2008
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| , resolution 2.85Å | |||||||
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| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
MS2-RNA HAIRPIN (4ONE-5) COMPLEX
OverviewOverview
The MS2 coat protein binds specifically to an RNA hairpin formed within the viral genome. By soaking different RNA fragments into crystals of MS2 coat protein capsids it is possible to determine the X-ray structure of the RNA-protein complexes formed. Here we present the structure to 2.85 A resolution of a complex between a chemically modified RNA hairpin variant and the MS2 coat protein. This RNA variant has a substitution at the -5 base position, which has been shown previously to be pyrimidine-specific and is a uracil in the wild-type RNA. The modified RNA hairpin contains a pyridin-4-one base (4one) at this position that lacks the exocyclic 2-oxygen eliminating the possibility of forming a hydrogen bond to asparagine A87 in the protein. The 4one complex structure shows an unprecedented major conformational change in the loop region of the RNA, whereas there is almost no change in the conformation of the protein.
About this StructureAbout this Structure
2IZ9 is a Single protein structure of sequence from Enterobacterio phage ms2. This structure supersedes the now removed PDB entry 1DZS. Full crystallographic information is available from OCA.
ReferenceReference
Deletion of a single hydrogen bonding atom from the MS2 RNA operator leads to dramatic rearrangements at the RNA-coat protein interface., Grahn E, Stonehouse NJ, Adams CJ, Fridborg K, Beigelman L, Matulic-Adamic J, Warriner SL, Stockley PG, Liljas L, Nucleic Acids Res. 2000 Dec 1;28(23):4611-6. PMID:11095669
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