2iwm: Difference between revisions
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2pva|2PVA]], [[3pva|3PVA]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iwm OCA], [http://www.ebi.ac.uk/pdbsum/2iwm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iwm RCSB]</span> | |||
}} | }} | ||
| Line 33: | Line 36: | ||
[[Category: zymogen]] | [[Category: zymogen]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:49:40 2008'' | ||
Revision as of 03:49, 31 March 2008
| |||||||
| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Penicillin amidase, with EC number 3.5.1.11 | ||||||
| Related: | 2PVA, 3PVA
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS
OverviewOverview
The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme.
About this StructureAbout this Structure
2IWM is a Single protein structure of sequence from Lysinibacillus sphaericus. Full crystallographic information is available from OCA.
ReferenceReference
Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:16508111
Page seeded by OCA on Mon Mar 31 03:49:40 2008