2dg0: Difference between revisions
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==Crystal structure of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus== | ==Crystal structure of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus== | ||
<StructureSection load='2dg0' size='340' side='right' caption='[[2dg0]], [[Resolution|resolution]] 2.40Å' scene=''> | <StructureSection load='2dg0' size='340' side='right' caption='[[2dg0]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2dg1|2dg1]], [[1v04|1v04]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2dg1|2dg1]], [[1v04|1v04]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dg0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dg0 OCA], [http://pdbe.org/2dg0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2dg0 RCSB], [http://www.ebi.ac.uk/pdbsum/2dg0 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dg0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dg0 OCA], [http://pdbe.org/2dg0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2dg0 RCSB], [http://www.ebi.ac.uk/pdbsum/2dg0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2dg0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dg/2dg0_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dg/2dg0_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
Revision as of 09:25, 30 May 2018
Crystal structure of Drp35, a 35kDa drug responsive protein from Staphylococcus aureusCrystal structure of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedDrp35 is a protein induced by cell wall-affecting antibiotics or detergents; it possesses calcium-dependent lactonase activity. To determine the molecular basis of the lactonase activity, we first solved the crystal structures of Drp35 with and without Ca(2+); these showed that the molecule has a six-bladed beta-propeller structure with two calcium ions bound at the center of the beta-propeller and surface region. Mutational analyses of evolutionarily conserved residues revealed that the central calcium-binding site is essential for the enzymatic activity of Drp35. Substitution of some other amino acid residues for the calcium-binding residues demonstrated the critical contributions of Glu(48), Asp(138), and Asp(236) to the enzymatic activity. Differential scanning calorimetric analysis revealed that the loss of activity of E48Q and D236N, but not D138N, was attributed to their inability to hold the calcium ion. Further structural analysis of the D138N mutant indicates that it lacks a water molecule bound to the calcium ion rather than the calcium ion itself. Based on these observations and structural information, a possible catalytic mechanism in which the calcium ion and its binding residues play direct roles was proposed for the lactonase activity of Drp35. Structural and mutational analyses of Drp35 from Staphylococcus aureus: a possible mechanism for its lactonase activity.,Tanaka Y, Morikawa K, Ohki Y, Yao M, Tsumoto K, Watanabe N, Ohta T, Tanaka I J Biol Chem. 2007 Feb 23;282(8):5770-80. Epub 2006 Dec 13. PMID:17166853[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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