6c5y: Difference between revisions
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==Crystal structure of thaumatin from microcrystals== | |||
<StructureSection load='6c5y' size='340' side='right' caption='[[6c5y]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6c5y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C5Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C5Y FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c5y OCA], [http://pdbe.org/6c5y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c5y RCSB], [http://www.ebi.ac.uk/pdbsum/6c5y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c5y ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/THM1_THADA THM1_THADA]] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 microm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines. | |||
Sample manipulation and data assembly for robust microcrystal synchrotron crystallography.,Guo G, Fuchs MR, Shi W, Skinner J, Berman E, Ogata CM, Hendrickson WA, McSweeney S, Liu Q IUCrJ. 2018 Apr 19;5(Pt 3):238-246. doi: 10.1107/S2052252518005389. eCollection, 2018 May 1. PMID:29755741<ref>PMID:29755741</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 6c5y" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Berman, E]] | |||
[[Category: Fuchs, M]] | [[Category: Fuchs, M]] | ||
[[Category: Guo, G]] | [[Category: Guo, G]] | ||
[[Category: Hendrickson, W A]] | |||
[[Category: Liu, Q]] | |||
[[Category: McSweeney, S]] | |||
[[Category: Ogata, C M]] | |||
[[Category: Shi, W]] | [[Category: Shi, W]] | ||
[[Category: Skinner, J]] | [[Category: Skinner, J]] | ||
[[Category: Data analysis]] | |||
[[Category: Microcrystal]] | |||
[[Category: Microdiffraction]] | |||
[[Category: Plant protein]] | |||
Revision as of 08:33, 30 May 2018
Crystal structure of thaumatin from microcrystalsCrystal structure of thaumatin from microcrystals
Structural highlights
Function[THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis. Publication Abstract from PubMedWith the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 microm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines. Sample manipulation and data assembly for robust microcrystal synchrotron crystallography.,Guo G, Fuchs MR, Shi W, Skinner J, Berman E, Ogata CM, Hendrickson WA, McSweeney S, Liu Q IUCrJ. 2018 Apr 19;5(Pt 3):238-246. doi: 10.1107/S2052252518005389. eCollection, 2018 May 1. PMID:29755741[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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