2irv: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 2irv |SIZE=350|CAPTION= <scene name='initialview01'>2irv</scene>, resolution 2.3Å | |PDB= 2irv |SIZE=350|CAPTION= <scene name='initialview01'>2irv</scene>, resolution 2.3Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PGV:(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL+(11E)-OCTADEC-11-ENOATE'>PGV</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= glpG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= glpG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2ic8|2IC8]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2irv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2irv OCA], [http://www.ebi.ac.uk/pdbsum/2irv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2irv RCSB]</span> | |||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Bibi, E.]] | [[Category: Bibi, E.]] | ||
[[Category: Fass, D.]] | [[Category: Fass, D.]] | ||
[[Category: cavity]] | [[Category: cavity]] | ||
[[Category: membrane protein]] | [[Category: membrane protein]] | ||
[[Category: ser-his dyad]] | [[Category: ser-his dyad]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:47:40 2008'' | ||
Revision as of 03:47, 31 March 2008
| |||||||
| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | glpG (Escherichia coli) | ||||||
| Related: | 2IC8
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of GlpG, a rhomboid intramembrane serine protease
OverviewOverview
Intramembrane proteases catalyze peptide bond cleavage of integral membrane protein substrates. This activity is crucial for many biological and pathological processes. Rhomboids are evolutionarily widespread intramembrane serine proteases. Here, we present the 2.3-A-resolution crystal structure of a rhomboid from Escherichia coli. The enzyme has six transmembrane helices, five of which surround a short TM4, which starts deep within the membrane at the catalytic serine residue. Thus, the catalytic serine is in an externally exposed cavity, which provides a hydrophilic environment for proteolysis. Our results reveal a mechanism to enable water-dependent catalysis at the depth of the hydrophobic milieu of the membrane and suggest how substrates gain access to the sequestered rhomboid active site.
About this StructureAbout this Structure
2IRV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for intramembrane proteolysis by rhomboid serine proteases., Ben-Shem A, Fass D, Bibi E, Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):462-6. Epub 2006 Dec 26. PMID:17190827
Page seeded by OCA on Mon Mar 31 03:47:40 2008