2ci6: Difference between revisions
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== | |||
==Crystal Structure of Dimethylarginine dimethylaminohydrolase I bound with Zinc low pH== | |||
<StructureSection load='2ci6' size='340' side='right' caption='[[2ci6]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='2ci6' size='340' side='right' caption='[[2ci6]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2c6z|2c6z]], [[2ci1|2ci1]], [[2ci3|2ci3]], [[2ci4|2ci4]], [[2ci5|2ci5]], [[2ci7|2ci7]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2c6z|2c6z]], [[2ci1|2ci1]], [[2ci3|2ci3]], [[2ci4|2ci4]], [[2ci5|2ci5]], [[2ci7|2ci7]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylargininase Dimethylargininase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.18 3.5.3.18] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylargininase Dimethylargininase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.18 3.5.3.18] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ci6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ci6 OCA], [http://pdbe.org/2ci6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ci6 RCSB], [http://www.ebi.ac.uk/pdbsum/2ci6 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ci6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ci6 OCA], [http://pdbe.org/2ci6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ci6 RCSB], [http://www.ebi.ac.uk/pdbsum/2ci6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ci6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/2ci6_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/2ci6_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ci6 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 2ci6" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2ci6" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Dimethylarginine Dimethylaminohydrolase|Dimethylarginine Dimethylaminohydrolase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Grutter, M G]] | [[Category: Grutter, M G]] | ||
[[Category: Vasak, M]] | [[Category: Vasak, M]] | ||
[[Category: Acetylation]] | |||
[[Category: Adma]] | [[Category: Adma]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
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[[Category: Nos regulation]] | [[Category: Nos regulation]] | ||
[[Category: S-nitrosylation]] | [[Category: S-nitrosylation]] | ||
[[Category: Zinc]] | |||
Revision as of 10:59, 23 May 2018
Crystal Structure of Dimethylarginine dimethylaminohydrolase I bound with Zinc low pHCrystal Structure of Dimethylarginine dimethylaminohydrolase I bound with Zinc low pH
Structural highlights
Function[DDAH1_BOVIN] Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedDimethylarginine dimethylaminohydrolase (DDAH) is involved in the regulation of nitric oxide synthase (NOS) by metabolizing the free endogenous arginine derivatives N(omega)-methyl-L-arginine (MMA) and N(omega),N(omega)-dimethyl-L-arginine (ADMA), which are competitive inhibitors of NOS. Here, we present high-resolution crystal structures of DDAH isoform 1 (DDAH-1) isolated from bovine brain in complex with different inhibitors, including S-nitroso-L-homocysteine and Zn2+, a regulator of this mammalian enzyme. The structure of DDAH-1 consists of a propeller-like fold similar to other arginine-modifying enzymes and a flexible loop, which adopts different conformations and acts as a lid at the entrance of the active site. The orientation and interaction mode of inhibitors in the active site give insight into the regulation and the molecular mechanism of the enzyme. The presented structures provide a basis for the structure-based development of specific DDAH-1 inhibitors that might be useful in the therapeutic treatment of NOS dysfunction-related diseases. Structure of the mammalian NOS regulator dimethylarginine dimethylaminohydrolase: A basis for the design of specific inhibitors.,Frey D, Braun O, Briand C, Vasak M, Grutter MG Structure. 2006 May;14(5):901-11. PMID:16698551[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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