2i24: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 2i24 |SIZE=350|CAPTION= <scene name='initialview01'>2i24</scene>, resolution 1.35Å | |PDB= 2i24 |SIZE=350|CAPTION= <scene name='initialview01'>2i24</scene>, resolution 1.35Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2i25|2I25]], [[2i26|2I26]], [[2i27|2I27]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i24 OCA], [http://www.ebi.ac.uk/pdbsum/2i24 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i24 RCSB]</span> | |||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Stanfield, R L.]] | [[Category: Stanfield, R L.]] | ||
[[Category: Wilson, I A.]] | [[Category: Wilson, I A.]] | ||
[[Category: immunoglobulin fold]] | [[Category: immunoglobulin fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:38:39 2008'' | ||
Revision as of 03:38, 31 March 2008
| |||||||
| , resolution 1.35Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Related: | 2I25, 2I26, 2I27
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure analysis of the nurse shark New Antigen Receptor PBLA8 variable domain
OverviewOverview
Sharks express an unusual heavy-chain isotype called IgNAR, whose variable regions bind antigen as independent soluble domains. To further probe affinity maturation of the IgNAR response, we structurally characterized the germline and somatically matured versions of a type II variable (V) region, both in the presence and absence of its antigen, hen egg-white lysozyme. Despite a disulfide bond linking complementarity determining regions (CDRs) 1 and 3, both germline and somatically matured V regions displayed significant structural changes in these CDRs upon complex formation with antigen. Somatic mutations in the IgNAR V region serve to increase the number of contacts with antigen, as reflected by a tenfold increase in affinity, and one of these mutations appears to stabilize the CDR3 region. In addition, a residue in the HV4 loop plays an important role in antibody-antigen interaction, consistent with the high rate of somatic mutations in this non-CDR loop.
About this StructureAbout this Structure
2I24 is a Protein complex structure of sequences from Ginglymostoma cirratum. Full crystallographic information is available from OCA.
ReferenceReference
Maturation of shark single-domain (IgNAR) antibodies: evidence for induced-fit binding., Stanfield RL, Dooley H, Verdino P, Flajnik MF, Wilson IA, J Mol Biol. 2007 Mar 23;367(2):358-72. Epub 2006 Dec 22. PMID:17258766
Page seeded by OCA on Mon Mar 31 03:38:39 2008