2hv8: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 2hv8 |SIZE=350|CAPTION= <scene name='initialview01'>2hv8</scene>, resolution 1.86Å | |PDB= 2hv8 |SIZE=350|CAPTION= <scene name='initialview01'>2hv8</scene>, resolution 1.86Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=2ME:METHOXYETHANE'>2ME</scene>, <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=2ME:METHOXYETHANE'>2ME</scene>, <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= RAB11A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RAB11FIP3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= RAB11A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RAB11FIP3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hv8 OCA], [http://www.ebi.ac.uk/pdbsum/2hv8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hv8 RCSB]</span> | |||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Mishra, A.]] | [[Category: Mishra, A.]] | ||
[[Category: Prekeris, R.]] | [[Category: Prekeris, R.]] | ||
[[Category: cytokinesis]] | [[Category: cytokinesis]] | ||
[[Category: fip3]] | [[Category: fip3]] | ||
| Line 37: | Line 35: | ||
[[Category: recycling endosome]] | [[Category: recycling endosome]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:35:52 2008'' | ||
Revision as of 03:35, 31 March 2008
| |||||||
| , resolution 1.86Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Gene: | RAB11A (Homo sapiens), RAB11FIP3 (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of GTP-bound Rab11 in complex with FIP3
OverviewOverview
The Rab11 GTPase regulates recycling of internalized plasma membrane receptors and is essential for completion of cytokinesis. A family of Rab11 interacting proteins (FIPs) that conserve a C-terminal Rab-binding domain (RBD) selectively recognize the active form of Rab11. Normal completion of cytokinesis requires a complex between Rab11 and FIP3. Here, we report the crystal structure and mutational analysis of a heterotetrameric complex between constitutively active Rab11 and a FIP3 construct that includes the RBD. Two Rab11 molecules bind to dyad symmetric sites at the C terminus of FIP3, which forms a non-canonical coiled-coiled dimer with a flared C terminus and hook region. The RBD overlaps with the coiled coil and extends through the C-terminal hook. Although FIP3 engages the switch and interswitch regions of Rab11, the mode of interaction differs significantly from that of other Rab-effector complexes. In particular, the switch II region undergoes a large structural rearrangement from an ordered but non-complementary active conformation to a remodeled conformation that facilitates the interaction with FIP3. Finally, we provide evidence that FIP3 can form homo-oligomers in cells, and that a critical determinant of Rab11 binding in vitro is necessary for FIP3 recruitment to recycling endosomes during cytokinesis.
About this StructureAbout this Structure
2HV8 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for Rab11-mediated recruitment of FIP3 to recycling endosomes., Eathiraj S, Mishra A, Prekeris R, Lambright DG, J Mol Biol. 2006 Nov 24;364(2):121-35. Epub 2006 Aug 26. PMID:17007872
Page seeded by OCA on Mon Mar 31 03:35:52 2008