DNA polymerase: Difference between revisions
Cori Damron (talk | contribs) No edit summary |
Cori Damron (talk | contribs) No edit summary |
||
| Line 107: | Line 107: | ||
==Structure== | ==Structure== | ||
The basic structure of all DNA polymerases consists of subdomains referred to as the palm, fingers, and thumb. The palm contains catalytically essential amino acids in it's active sites. The fingers are essential for nucleotide recognition and binding. The thumb is important for the binding of the DNA substrate. These subdomains, along with other subdomains specific to each family, are essential for the correct functioning of DNA polymerase. | The basic structure of all DNA polymerases consists of subdomains referred to as the palm, fingers, and thumb. The palm contains catalytically essential amino acids in it's active sites. The fingers are essential for nucleotide recognition and binding. The thumb is important for the binding of the DNA substrate. These subdomains, along with other subdomains specific to each family, are essential for the correct functioning of DNA polymerase. The structures of each of these subdomains are slightly different for each polymerase, but not much is known about those subtle differences. <ref name="structure">PMID: 16230118</ref> | ||
===Family A=== | ===Family A=== | ||
In addition to the basic structure of DNA polymerase, the Family A polymerases also have a 5'-3' exonuclease that is required for the removal of RNA primers from Okazaki fragments. Not all, but some Family A polymerases also a 3'-5' exonuclease that is responsible for proofreading the DNA. <ref name="structure" | In addition to the basic structure of DNA polymerase, the Family A polymerases also have a 5'-3' exonuclease that is required for the removal of RNA primers from Okazaki fragments. Not all, but some Family A polymerases also a 3'-5' exonuclease that is responsible for proofreading the DNA. <ref name="structure" /> | ||
===Family B=== | ===Family B=== | ||