1z7z: Difference between revisions
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==Cryo-em structure of human coxsackievirus A21 complexed with five domain icam-1kilifi== | ==Cryo-em structure of human coxsackievirus A21 complexed with five domain icam-1kilifi== | ||
<StructureSection load='1z7z' size='340' side='right' caption='[[1z7z]], [[Resolution|resolution]] 8.00Å' scene=''> | <StructureSection load='1z7z' size='340' side='right' caption='[[1z7z]], [[Resolution|resolution]] 8.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1z7z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[1z7z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_coxsackievirus_a21 Human coxsackievirus a21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z7Z FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1iam|1iam]], [[1z7s|1z7s]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1iam|1iam]], [[1z7s|1z7s]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7z OCA], [http://pdbe.org/1z7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z7z RCSB], [http://www.ebi.ac.uk/pdbsum/1z7z PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z7z OCA], [http://pdbe.org/1z7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z7z RCSB], [http://www.ebi.ac.uk/pdbsum/1z7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z7z ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z7/1z7z_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z7/1z7z_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1z7z" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1z7z" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Human coxsackievirus a21]] | |||
[[Category: Bator-Kelly, C M]] | [[Category: Bator-Kelly, C M]] | ||
[[Category: Chipman, P R]] | [[Category: Chipman, P R]] | ||
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[[Category: Xiao, C]] | [[Category: Xiao, C]] | ||
[[Category: Cd54]] | [[Category: Cd54]] | ||
[[Category: Icam-1]] | [[Category: Icam-1]] | ||
[[Category: Icosahedral virus]] | [[Category: Icosahedral virus]] | ||
[[Category: Kilifi]] | [[Category: Kilifi]] | ||
[[Category: Virus-receptor complex]] | [[Category: Virus-receptor complex]] | ||
Revision as of 11:54, 2 May 2018
Cryo-em structure of human coxsackievirus A21 complexed with five domain icam-1kilifiCryo-em structure of human coxsackievirus A21 complexed with five domain icam-1kilifi
Structural highlights
Function[ICAM1_HUMAN] ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.[1] [2] [3] [4] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedCVA21 and polioviruses both belong to the Enterovirus genus in the family of Picornaviridae, whereas rhinoviruses form a distinct picornavirus genus. Nevertheless, CVA21 and the major group of human rhinoviruses recognize intercellular adhesion molecule-1 (ICAM-1) as their cellular receptor, whereas polioviruses use poliovirus receptor. The crystal structure of CVA21 has been determined to 3.2 A resolution. Its structure has greater similarity to poliovirus structures than to other known picornavirus structures. Cryo-electron microscopy (cryo-EM) was used to determine an 8.0 A resolution structure of CVA21 complexed with an ICAM-1 variant, ICAM-1(Kilifi). The cryo-EM map was fitted with the crystal structures of ICAM-1 and CVA21. Significant differences in the structure of CVA21 with respect to the poliovirus structures account for the inability of ICAM-1 to bind polioviruses. The interface between CVA21 and ICAM-1 has shape and electrostatic complementarity with many residues being conserved among those CVAs that bind ICAM-1. The crystal structure of coxsackievirus A21 and its interaction with ICAM-1.,Xiao C, Bator-Kelly CM, Rieder E, Chipman PR, Craig A, Kuhn RJ, Wimmer E, Rossmann MG Structure. 2005 Jul;13(7):1019-33. PMID:16004874[5] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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