2hil: Difference between revisions
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|PDB= 2hil |SIZE=350|CAPTION= <scene name='initialview01'>2hil</scene>, resolution 12.5Å | |PDB= 2hil |SIZE=350|CAPTION= <scene name='initialview01'>2hil</scene>, resolution 12.5Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=OPE:PHOSPHORIC ACID MONO-(2-AMINO-ETHYL) ESTER'>OPE</scene> | |LIGAND= <scene name='pdbligand=DT6:2,4-BIS(ACETYLAMINO)-1,5-ANHYDRO-2,4-DIDEOXY-D-GLUCITOL'>DT6</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=OPE:PHOSPHORIC+ACID+MONO-(2-AMINO-ETHYL)+ESTER'>OPE</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2hi2|2HI2]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hil OCA], [http://www.ebi.ac.uk/pdbsum/2hil PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hil RCSB]</span> | |||
}} | }} | ||
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[[Category: Tainer, J A.]] | [[Category: Tainer, J A.]] | ||
[[Category: Volkmann, N.]] | [[Category: Volkmann, N.]] | ||
[[Category: 3d image reconstruction]] | [[Category: 3d image reconstruction]] | ||
[[Category: antigenic variation]] | [[Category: antigenic variation]] | ||
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[[Category: virulence factor]] | [[Category: virulence factor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:30:56 2008'' | ||
Revision as of 03:30, 31 March 2008
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| , resolution 12.5Å | |||||||
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| Ligands: | , , | ||||||
| Related: | 2HI2
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the Neisseria gonorrhoeae Type IV pilus filament from x-ray crystallography and electron cryomicroscopy
OverviewOverview
Type IV pili (T4P) are long, thin, flexible filaments on bacteria that undergo assembly-disassembly from inner membrane pilin subunits and exhibit astonishing multifunctionality. Neisseria gonorrhoeae (gonococcal or GC) T4P are prototypic virulence factors and immune targets for increasingly antibiotic-resistant human pathogens, yet detailed structures are unavailable for any T4P. Here, we determined a detailed experimental GC-T4P structure by quantitative fitting of a 2.3 A full-length pilin crystal structure into a 12.5 A resolution native GC-T4P reconstruction solved by cryo-electron microscopy (cryo-EM) and iterative helical real space reconstruction. Spiraling three-helix bundles form the filament core, anchor the globular heads, and provide strength and flexibility. Protruding hypervariable loops and posttranslational modifications in the globular head shield conserved functional residues in pronounced grooves, creating a surprisingly corrugated pilus surface. These results clarify T4P multifunctionality and assembly-disassembly while suggesting unified assembly mechanisms for T4P, archaeal flagella, and type II secretion system filaments.
About this StructureAbout this Structure
2HIL is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions., Craig L, Volkmann N, Arvai AS, Pique ME, Yeager M, Egelman EH, Tainer JA, Mol Cell. 2006 Sep 1;23(5):651-62. PMID:16949362
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