6ehf: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==OmpT (in-vitro folded), an outer membrane protein Vibrio cholerae (trimer form)== | |||
<StructureSection load='6ehf' size='340' side='right' caption='[[6ehf]], [[Resolution|resolution]] 2.72Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ehf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EHF FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ehf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ehf OCA], [http://pdbe.org/6ehf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ehf RCSB], [http://www.ebi.ac.uk/pdbsum/6ehf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ehf ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The outer membranes (OM) of many Gram-negative bacteria contain general porins, which form nonspecific, large-diameter channels for the diffusional uptake of small molecules required for cell growth and function. While the porins of Enterobacteriaceae (e.g., E. coli OmpF and OmpC) have been extensively characterized structurally and biochemically, much less is known about their counterparts in Vibrionaceae. Vibrio cholerae, the causative agent of cholera, has two major porins, OmpU and OmpT, for which no structural information is available despite their importance for the bacterium. Here we report high-resolution X-ray crystal structures of V. cholerae OmpU and OmpT complemented with molecular dynamics simulations. While similar overall to other general porins, the channels of OmpU and OmpT have unusual constrictions that create narrower barriers for small-molecule permeation and change the internal electric fields of the channels. Together with electrophysiological and in vitro transport data, our results illuminate small-molecule uptake within the Vibrionaceae. | |||
Unusual Constriction Zones in the Major Porins OmpU and OmpT from Vibrio cholerae.,Pathania M, Acosta-Gutierrez S, Bhamidimarri SP, Basle A, Winterhalter M, Ceccarelli M, van den Berg B Structure. 2018 Apr 3. pii: S0969-2126(18)30091-1. doi:, 10.1016/j.str.2018.03.010. PMID:29657131<ref>PMID:29657131</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6ehf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Pathania, M]] | |||
[[Category: Berg, B van den]] | |||
[[Category: Diffusion channel]] | |||
[[Category: Diffusion porin]] | |||
[[Category: Ion-channel]] | |||
[[Category: Ion-transport]] | |||
[[Category: Membrane beta barrel]] | |||
[[Category: Membrane protein]] | |||
[[Category: Non-specific porin]] | |||
[[Category: Ompf or ompc ortholog]] | |||
[[Category: Outer membrane protein]] | |||
[[Category: Porin]] | |||
Revision as of 08:36, 25 April 2018
OmpT (in-vitro folded), an outer membrane protein Vibrio cholerae (trimer form)OmpT (in-vitro folded), an outer membrane protein Vibrio cholerae (trimer form)
Structural highlights
Publication Abstract from PubMedThe outer membranes (OM) of many Gram-negative bacteria contain general porins, which form nonspecific, large-diameter channels for the diffusional uptake of small molecules required for cell growth and function. While the porins of Enterobacteriaceae (e.g., E. coli OmpF and OmpC) have been extensively characterized structurally and biochemically, much less is known about their counterparts in Vibrionaceae. Vibrio cholerae, the causative agent of cholera, has two major porins, OmpU and OmpT, for which no structural information is available despite their importance for the bacterium. Here we report high-resolution X-ray crystal structures of V. cholerae OmpU and OmpT complemented with molecular dynamics simulations. While similar overall to other general porins, the channels of OmpU and OmpT have unusual constrictions that create narrower barriers for small-molecule permeation and change the internal electric fields of the channels. Together with electrophysiological and in vitro transport data, our results illuminate small-molecule uptake within the Vibrionaceae. Unusual Constriction Zones in the Major Porins OmpU and OmpT from Vibrio cholerae.,Pathania M, Acosta-Gutierrez S, Bhamidimarri SP, Basle A, Winterhalter M, Ceccarelli M, van den Berg B Structure. 2018 Apr 3. pii: S0969-2126(18)30091-1. doi:, 10.1016/j.str.2018.03.010. PMID:29657131[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||