3hb4: Difference between revisions
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==17beta-hydroxysteroid dehydrogenase type1 complexed with E2B== | ==17beta-hydroxysteroid dehydrogenase type1 complexed with E2B== | ||
<StructureSection load='3hb4' size='340' side='right' caption='[[3hb4]], [[Resolution|resolution]] 2.21Å' scene=''> | <StructureSection load='3hb4' size='340' side='right' caption='[[3hb4]], [[Resolution|resolution]] 2.21Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hb5|3hb5]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hb5|3hb5]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/17-beta-estradiol_17-dehydrogenase 17-beta-estradiol 17-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.62 1.1.1.62] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/17-beta-estradiol_17-dehydrogenase 17-beta-estradiol 17-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.62 1.1.1.62] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hb4 OCA], [http://pdbe.org/3hb4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hb4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hb4 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hb4 OCA], [http://pdbe.org/3hb4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hb4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hb4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hb4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hb/3hb4_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hb/3hb4_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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[[Category: Poirier, D]] | [[Category: Poirier, D]] | ||
[[Category: Zhu, D W]] | [[Category: Zhu, D W]] | ||
[[Category: Cytoplasm]] | |||
[[Category: Lipid synthesis]] | [[Category: Lipid synthesis]] | ||
[[Category: Nadp]] | [[Category: Nadp]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Polymorphism]] | |||
[[Category: Steroid biosynthesis]] | [[Category: Steroid biosynthesis]] | ||
Revision as of 09:32, 4 April 2018
17beta-hydroxysteroid dehydrogenase type1 complexed with E2B17beta-hydroxysteroid dehydrogenase type1 complexed with E2B
Structural highlights
Function[DHB1_HUMAN] Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedOestradiol is a well-characterized sex hormone that stimulates breast cancer and other oestrogen-related diseases. 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) catalyses the last step in the synthesis of oestradiol and androstenediol in breast tumour tissue. The enzyme's high expression and activity after simultaneous blockade of oestrogen receptors and inhibition of aromatase in the tumour shows the necessity for its inhibition as a requirement for breast cancer therapy. In the present paper, we report structures of the binary and ternary complexes of 17beta-HSD1 with a new inhibitor E2B {3-[3',17'beta-dihydroxyestra-1',3',5'(10')-trien-16'beta-methyl]benzamide }, and the enzyme inhibition by the later. The IC50 value for E2B was determined to be 42 nM in T47D cells. Multiple interactions between E2B and the enzyme include hydrogen bonds and hydrophobic interactions, as well as pi-pi interactions. A kinetic study demonstrated that E2B inhibits the enzyme's reduction forming oestradiol from oestrone, with a Ki of 0.9+/-0.15 nM. Such strong inhibition is in agreement with its extensive interaction with the enzyme, suggesting its potential as a lead compound for breast cancer therapy. In fact, this possibility is enhanced by its capacity for cell penetration similar to natural steroids. Such inhibitors that block oestrogen synthesis to suppress the sulfatase pathway producing oestradiol can be used in adjuvant therapies with oestrogen receptor blockade, opening a new orientation of breast cancer treatment. Binary and ternary crystal structure analyses of a novel inhibitor with 17beta-HSD type 1: a lead compound for breast cancer therapy.,Mazumdar M, Fournier D, Zhu DW, Cadot C, Poirier D, Lin SX Biochem J. 2009 Dec 10;424(3):357-66. PMID:19929851[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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