6bp8: Difference between revisions
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==Recombinant major vault protein [Rattus norvegicus] structure in solution: conformation 1== | |||
<StructureSection load='6bp8' size='340' side='right' caption='[[6bp8]], [[Resolution|resolution]] 4.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6bp8]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BP8 FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6bp7|6bp7]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bp8 OCA], [http://pdbe.org/6bp8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bp8 RCSB], [http://www.ebi.ac.uk/pdbsum/6bp8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bp8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/MVP_RAT MVP_RAT]] Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Prior crystal structures of the vault have provided clues of its structural variability but are non-conclusive due to crystal packing. Here, we obtained vaults by engineering at the N terminus of rat major vault protein (MVP) an HIV-1 Gag protein segment and determined their near-atomic resolution ( approximately 4.8 A) structures in a solution/non-crystalline environment. The barrel-shaped vaults in solution adopt two conformations, 1 and 2, both with D39 symmetry. From the N to C termini, each MVP monomer has three regions: body, shoulder, and cap. While conformation 1 is identical to one of the crystal structures, the shoulder in conformation 2 is translocated longitudinally up to 10 A, resulting in an outward-projected cap. Our structures clarify the structural discrepancies in the body region in the prior crystallography models. The vault's drug-delivery potential is highlighted by the internal disposition and structural flexibility of its Gag-loaded N-terminal extension at the barrel waist of the engineered vault. | |||
Solution Structures of Engineered Vault Particles.,Ding K, Zhang X, Mrazek J, Kickhoefer VA, Lai M, Ng HL, Yang OO, Rome LH, Zhou ZH Structure. 2018 Mar 7. pii: S0969-2126(18)30054-6. doi:, 10.1016/j.str.2018.02.014. PMID:29551289<ref>PMID:29551289</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6bp8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Ding, K]] | |||
[[Category: Kickhoefer, V A]] | |||
[[Category: Lai, M]] | |||
[[Category: Mrazek, J]] | |||
[[Category: Ng, H L]] | |||
[[Category: Rome, L H]] | |||
[[Category: Yang, O O]] | |||
[[Category: Zhang, X]] | |||
[[Category: Zhou, Z H]] | |||
[[Category: Structural protein]] | |||
[[Category: Vault recombinant protein structure engineered nano-particle]] | |||
Revision as of 08:57, 4 April 2018
Recombinant major vault protein [Rattus norvegicus] structure in solution: conformation 1Recombinant major vault protein [Rattus norvegicus] structure in solution: conformation 1
Structural highlights
Function[MVP_RAT] Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases (By similarity). Publication Abstract from PubMedPrior crystal structures of the vault have provided clues of its structural variability but are non-conclusive due to crystal packing. Here, we obtained vaults by engineering at the N terminus of rat major vault protein (MVP) an HIV-1 Gag protein segment and determined their near-atomic resolution ( approximately 4.8 A) structures in a solution/non-crystalline environment. The barrel-shaped vaults in solution adopt two conformations, 1 and 2, both with D39 symmetry. From the N to C termini, each MVP monomer has three regions: body, shoulder, and cap. While conformation 1 is identical to one of the crystal structures, the shoulder in conformation 2 is translocated longitudinally up to 10 A, resulting in an outward-projected cap. Our structures clarify the structural discrepancies in the body region in the prior crystallography models. The vault's drug-delivery potential is highlighted by the internal disposition and structural flexibility of its Gag-loaded N-terminal extension at the barrel waist of the engineered vault. Solution Structures of Engineered Vault Particles.,Ding K, Zhang X, Mrazek J, Kickhoefer VA, Lai M, Ng HL, Yang OO, Rome LH, Zhou ZH Structure. 2018 Mar 7. pii: S0969-2126(18)30054-6. doi:, 10.1016/j.str.2018.02.014. PMID:29551289[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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