2gew: Difference between revisions
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|PDB= 2gew |SIZE=350|CAPTION= <scene name='initialview01'>2gew</scene>, resolution 0.97Å | |PDB= 2gew |SIZE=350|CAPTION= <scene name='initialview01'>2gew</scene>, resolution 0.97Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] </span> | ||
|GENE= choA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Streptomyces sp.]) | |GENE= choA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Streptomyces sp.]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gew OCA], [http://www.ebi.ac.uk/pdbsum/2gew PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gew RCSB]</span> | |||
}} | }} | ||
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[[Category: Lyubimov, A Y.]] | [[Category: Lyubimov, A Y.]] | ||
[[Category: Vrielink, A]] | [[Category: Vrielink, A]] | ||
[[Category: atomic resolution]] | [[Category: atomic resolution]] | ||
[[Category: flavoenzyme]] | [[Category: flavoenzyme]] | ||
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[[Category: steroid metabolism]] | [[Category: steroid metabolism]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:15:41 2008'' | ||
Revision as of 03:15, 31 March 2008
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| , resolution 0.97Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | choA (Streptomyces sp.) | ||||||
| Activity: | Cholesterol oxidase, with EC number 1.1.3.6 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Atomic resolution structure of cholesterol oxidase @ pH 9.0 (Streptomyces sp. SA-COO)
OverviewOverview
Hydrogen atoms are a vital component of enzyme structure and function. In recent years, atomic resolution crystallography (>or=1.2 A) has been successfully used to investigate the role of the hydrogen atom in enzymatic catalysis. Here, atomic resolution crystallography was used to study the effect of pH on cholesterol oxidase from Streptomyces sp., a flavoenzyme oxidoreductase. Crystallographic observations of the anionic oxidized flavin cofactor at basic pH are consistent with the UV-visible absorption profile of the enzyme and readily explain the reversible pH-dependent loss of oxidation activity. Furthermore, a hydrogen atom, positioned at an unusually short distance from the main chain carbonyl oxygen of Met122 at high pH, was observed, suggesting a previously unknown mechanism of cofactor stabilization. This study shows how a redox active site responds to changes in the enzyme's environment and how these changes are able to influence the mechanism of enzymatic catalysis.
About this StructureAbout this Structure
2GEW is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.
ReferenceReference
Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment., Lyubimov AY, Lario PI, Moustafa I, Vrielink A, Nat Chem Biol. 2006 May;2(5):259-64. Epub 2006 Apr 9. PMID:16604066
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