User:Mark Macbeth/Sandbox2: Difference between revisions

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=== Alternative Splicing Pathways ===

[[Image:Sxl mechanism alternativesplicing figure version2.jpg|300px|left|thumb| '''Figure 3.''' 2-dimensional representation of alternative splicing repression by Sxl on the ''tra'' and ''msl-2'' genes.]]

The alternative splicing pathways of Sxl differ, but both involve repression at the 3' splice site<ref name="Handa"/><ref name="Black">. The ''tra'' expression pathway only involves the 3' splice site, while the ''msl-2'' pathway involves both the 3' splice site and the 5' splice site. Both mechanisms cause U2AF binding downstream with lower affinity (Fig. 3)<ref name="Black"/>. U2AF is a more general splicing factor than Sxl, and prefers cytidine-containing poly-uridine pre-mRNA sequences, so Sxl binds to the guanosine-containing pre-mRNA with a 10<sup>4</sup>-fold greater affinity<ref name="Handa"/>.

The alternative splicing pathways of Sxl differ, but both involve repression at the 3' splice site<ref name="Handa"/><ref name="Black"/>. The ''tra'' expression pathway only involves the 3' splice site, while the ''msl-2'' pathway involves both the 3' splice site and the 5' splice site. Both mechanisms cause U2AF binding downstream with lower affinity (Fig. 3)<ref name="Black"/>. U2AF is a more general splicing factor than Sxl, and prefers cytidine-containing poly-uridine pre-mRNA sequences, so Sxl binds to the guanosine-containing pre-mRNA with a 10<sup>4</sup>-fold greater affinity<ref name="Handa"/>.

=== Autoregulation ===

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 === Alternative Splicing Pathways ===
 [[Image:Sxl mechanism alternativesplicing figure version2.jpg|300px|left|thumb| '''Figure 3.''' 2-dimensional representation of alternative splicing repression by Sxl on the ''tra'' and ''msl-2'' genes.]]
-The alternative splicing pathways of Sxl differ, but both involve repression at the 3' splice site<ref name="Handa"/><ref name="Black">. The ''tra'' expression pathway only involves the 3' splice site, while the ''msl-2'' pathway involves both the 3' splice site and the 5' splice site. Both mechanisms cause U2AF binding downstream with lower affinity (Fig. 3)<ref name="Black"/>. U2AF is a more general splicing factor than Sxl, and prefers cytidine-containing poly-uridine pre-mRNA sequences, so Sxl binds to the guanosine-containing pre-mRNA with a 10<sup>4</sup>-fold greater affinity<ref name="Handa"/>.
+The alternative splicing pathways of Sxl differ, but both involve repression at the 3' splice site<ref name="Handa"/><ref name="Black"/>. The ''tra'' expression pathway only involves the 3' splice site, while the ''msl-2'' pathway involves both the 3' splice site and the 5' splice site. Both mechanisms cause U2AF binding downstream with lower affinity (Fig. 3)<ref name="Black"/>. U2AF is a more general splicing factor than Sxl, and prefers cytidine-containing poly-uridine pre-mRNA sequences, so Sxl binds to the guanosine-containing pre-mRNA with a 10<sup>4</sup>-fold greater affinity<ref name="Handa"/>.
 === Autoregulation ===