1u3e: Difference between revisions
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==DNA binding and cleavage by the HNH homing endonuclease I-HmuI== | ==DNA binding and cleavage by the HNH homing endonuclease I-HmuI== | ||
<StructureSection load='1u3e' size='340' side='right' caption='[[1u3e]], [[Resolution|resolution]] 2.92Å' scene=''> | <StructureSection load='1u3e' size='340' side='right' caption='[[1u3e]], [[Resolution|resolution]] 2.92Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1i3j|1i3j]], [[1a73|1a73]], [[1fsj|1fsj]], [[1pt3|1pt3]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1i3j|1i3j]], [[1a73|1a73]], [[1fsj|1fsj]], [[1pt3|1pt3]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bacteriophage SP01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10685 BPSP1])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bacteriophage SP01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10685 BPSP1])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u3e OCA], [http://pdbe.org/1u3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u3e RCSB], [http://www.ebi.ac.uk/pdbsum/1u3e PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u3e OCA], [http://pdbe.org/1u3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u3e RCSB], [http://www.ebi.ac.uk/pdbsum/1u3e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u3e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u3/1u3e_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u3/1u3e_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
Revision as of 11:35, 21 March 2018
DNA binding and cleavage by the HNH homing endonuclease I-HmuIDNA binding and cleavage by the HNH homing endonuclease I-HmuI
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe structure of I-HmuI, which represents the last family of homing endonucleases without a defining crystallographic structure, has been determined in complex with its DNA target. A series of diverse protein structural domains and motifs, contacting sequential stretches of nucleotide bases, are distributed along the DNA target. I-HmuI contains an N-terminal domain with a DNA-binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease. The combination and exchange of these features between protein families indicates that the genetic mobility associated with homing endonucleases extends to the level of independent structural domains. I-HmuI provides an unambiguous structural connection between the His-Cys box endonucleases and the bacterial colicins, supporting the hypothesis that these enzymes diverged from a common ancestral nuclease. DNA binding and cleavage by the HNH homing endonuclease I-HmuI.,Shen BW, Landthaler M, Shub DA, Stoddard BL J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:15313606[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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