2fs3: Difference between revisions
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|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= 5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | |GENE= 5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37554 Enterobacteria phage HK97]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2frp|2FRP]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fs3 OCA], [http://www.ebi.ac.uk/pdbsum/2fs3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fs3 RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
2FS3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 2FS3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk97 Enterobacteria phage hk97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FS3 OCA]. | ||
==Reference== | ==Reference== | ||
Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17098191 17098191] | Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17098191 17098191] | ||
[[Category: Enterobacteria phage | [[Category: Enterobacteria phage hk97]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cheng, N.]] | [[Category: Cheng, N.]] | ||
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[[Category: hk97]] | [[Category: hk97]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:06:54 2008'' | ||
Revision as of 03:06, 31 March 2008
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| , resolution 4.200Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | 5 (Enterobacteria phage HK97) | ||||||
| Related: | 2FRP
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Bacteriophage HK97 K169Y Head I
OverviewOverview
Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.
About this StructureAbout this Structure
2FS3 is a Single protein structure of sequence from Enterobacteria phage hk97. Full crystallographic information is available from OCA.
ReferenceReference
Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:17098191
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