1uzv: Difference between revisions
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Revision as of 18:12, 5 November 2007
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HIGH AFFINITY FUCOSE BINDING OF PSEUDOMONAS AERUGINOSA LECTIN II: 1.0 A CRYSTAL STRUCTURE OF THE COMPLEX
OverviewOverview
PA-IIL is a fucose-binding lectin from Pseudomonas aeruginosa that is, closely related to the virulence factors of the bacterium. Previous, structural studies have revealed a new carbohydrate-binding mode with, direct involvement of two calcium ions (Mitchell E, Houles C, Sudakevitz, D, Wimmerova M, Gautier C, Perez S, Wu AM, Gilboa-Garber N, Imberty A., Structural basis for selective recognition of oligosaccharides from cystic, fibrosis patients by the lectin PA-IIL of Pseudomonas aeruginosa. Nat, Struct Biol 2002;9:918-921). A combination of thermodynamic, structural, and computational methods has been used to study the basis of the high, affinity for the monosaccharide ligand. A titration microcalorimetry study, indicated that the high affinity is enthalpy driven. The crystal structure, of the tetrameric PA-IIL in complex with fucose and calcium was refined to, 1.0 A resolution and, in combination with modeling, allowed a proposal to, be made for the hydrogen-bond network in the binding site. Calculations of, partial charges using ab initio computational chemistry methods indicated, that extensive delocalization of charges between the calcium ions, the, side chains of the protein-binding site and the carbohydrate ligand is, responsible for the high enthalpy of binding and therefore for the, unusually high affinity observed for this unique mode of carbohydrate, recognition.
About this StructureAbout this Structure
1UZV is a Single protein structure of sequence from Pseudomonas aeruginosa with FUC, SO4 and CA as ligands. Structure known Active Site: C1A. Full crystallographic information is available from OCA.
ReferenceReference
High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches., Mitchell EP, Sabin C, Snajdrova L, Pokorna M, Perret S, Gautier C, Hofr C, Gilboa-Garber N, Koca J, Wimmerova M, Imberty A, Proteins. 2005 Feb 15;58(3):735-46. PMID:15573375
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