Nucleoplasmin: Difference between revisions
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= orthologey in Homo sapiens = | = orthologey in Homo sapiens = | ||
the NP in Humans is made out of <scene name='46/467273/Dimer/1'>dimer</scene> while each <scene name='46/467273/Monomer/2'>monomer</scene> consisted of five chains. The structure remain similiar to the one in Xenopus but with a change in amino acids in its <scene name='46/467273/Core_domain/2'>core domain</scene>, Val insted of Ile. | the NP in Humans is made out of <scene name='46/467273/Dimer/1'>dimer</scene> while each <scene name='46/467273/Monomer/2'>monomer</scene> consisted of five chains. The structure remain similiar to the one in Xenopus but with a change in amino acids in its <scene name='46/467273/Core_domain/2'>core domain</scene>, Val insted of Ile. | ||
The Decamer bind H2A-H2B dimers and H3-H4 tetramers simultaneously, In the absence of histone tetramers the pentamer binds H2A-H2B and formes central hub. When H3-H4 tetramers are recruited this results in a functional dimerization of the complex, and the decamer being formed. | The Decamer bind H2A-H2B dimers and H3-H4 tetramers simultaneously, In the absence of histone tetramers the pentamer binds H2A-H2B and formes central hub. When H3-H4 tetramers are recruited this results in a functional dimerization of the complex, and the decamer being formed. | ||
The interaction between NP and H2A-H2B has a strong electrostatic component and the complex formation results in charge neutralization, which in turn stabilize it by diminishing the repulsion due to the proposed accumulation of negatively charged residues at the face of the NP pentamer. | |||
[[Image:Histone Storage and Nucleosome Assembly.jpg|thumb|Histone Storage and Nucleosome Assembly]] | [[Image:Histone Storage and Nucleosome Assembly.jpg|thumb|Histone Storage and Nucleosome Assembly]] | ||
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7. Shuchismita Dutta, Ildiko´ V. Akey,Colin Dingwall, Kari L. Hartman,Tom Laue, Robert T. Nolte,James F. Head, and Christopher W. Akey. The Crystal Structure of Nucleoplasmin-Core: | 7. Shuchismita Dutta, Ildiko´ V. Akey,Colin Dingwall, Kari L. Hartman,Tom Laue, Robert T. Nolte,James F. Head, and Christopher W. Akey. The Crystal Structure of Nucleoplasmin-Core: | ||
Implications for Histone Binding and Nucleosome Assembly. Molecular Cell, Vol. 8, 841–853, October, 2001 | Implications for Histone Binding and Nucleosome Assembly. Molecular Cell, Vol. 8, 841–853, October, 2001 | ||
8. Isbaal Ramos, Jaime Martín-Benito, Ron Finn, Laura Bretan˜, Kerman Aloria, Jesu´ s M. Arizmendi, Juan Ausio´, Arturo Muga, Jose´ M. Valpuesta, and Adelina Prado. Nucleoplasmin Binds Histone H2A-H2B Dimers through Its Distal Face. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 285, NO. 44, pp. 33771–33778, October 29, 2010 | |||