Alice Clark/ATPsynthase

/>

Protected Page: This page can be edited only by its author, but you are encouraged to copy it freely to other pages (use the View Source tab), and add your creative ideas. Please see Proteopedia:Policy and Help:Protected_Pages.

ATP SynthaseATP Synthase

Exploring in 3D - left mouse button to rotate the protein model. - middle mouse button or scroll wheel to zoom (option-click on a Mac) - right mouse button for more options and information (control-click on a Mac). Have a go yourself now ==> The synthesis of ATP involves a number of steps 1. Binding of ADP and the phosphate 2. Formation of the new high energy phosphate-phosphate bond 3. Releasing the newly made ATP These steps are performed by a tiny motor, found in the mitochondrial membrane, called ATP synthase - shown here in 3D. The ATP synthase molecule is built up of different proteins each with a seperate role The contains alpha (shown in light blue) and beta (shown in dark blue) chains. It also has the adenosine diphosphate (ADP) molecules and the adenosine triphosphate ATP . See if you can zoom in on the ATP identify the base, sugar and phosphates that make up these molecules. Q1: Where is the F1 region located within the ATP synthase, and what is its role? The rotates putting the motor into three different conformations as the ADP binds, the phosphate bond is formed and the ATP being released. The F0 is a circular rotor that is formed of alpha helices that sit in the mitochondrial membrane. The hydrogen ions travel around the circular F0 motor, and turning the rotor in the process, much like a water wheel. The hydrogens are passed alone a chain of aspartic acid residues (amino acids) in the F0 motor, and then transferred to an arginine amino acid. The arginine passes the hydrogen to the rotor, which turns all the way around. Then the hydrogen is then passed to the opposite side of the membrane. To the right is a structure of ATP synthase shown using the Jmol viewer within Proteopedia. Click '>here</scene> for a view where the different components are coloured differently? See how many you can identify Q2: How many ATP can you find? Q3: What is the location and role of the FO region? ATP synthase is an example of a molecular motor. Use the Structure to answer the questions below. Q4: How many phosphates does ATP have? Q5: How many phosphates does ADP have Key Terms Aspartic acid ATP AMP ATP synthase

spinqualitylabelsall models ATP Synthase (PDB entry 5lqz) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image