4d74: Difference between revisions
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<StructureSection load='4d74' size='340' side='right' caption='[[4d74]], [[Resolution|resolution]] 1.57Å' scene=''> | <StructureSection load='4d74' size='340' side='right' caption='[[4d74]], [[Resolution|resolution]] 1.57Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4d74]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D74 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4d74]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwae Erwae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D74 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Erwae]] | |||
[[Category: Protein-tyrosine-phosphatase]] | [[Category: Protein-tyrosine-phosphatase]] | ||
[[Category: Benini, S]] | [[Category: Benini, S]] | ||
Revision as of 08:48, 8 March 2018
1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI
Structural highlights
Function[AMSI_ERWAM] May function as a phosphatase required for amylovoran (an exopolysaccharide that functions as a virulence factor) production. Publication Abstract from PubMedAmsI is a low-molecular-weight protein tyrosine phosphatase that regulates the production of amylovoran in the Gram-negative bacterium Erwinia amylovora, a specific pathogen of rosaceous plants such as apple, pear and quince. Amylovoran is an exopolysaccharide that is necessary for successful infection. In order to shed light on AmsI, its structure was solved at 1.57 A resolution at the same pH as its highest measured activity (pH 5.5). In the active site, a water molecule, bridging between the catalytic Arg15 and the reaction-product analogue sulfate, might be representative of the water molecule attacking the phospho-cysteine intermediate in the second step of the reaction mechanism. Characterization and 1.57 A resolution structure of the key fire blight phosphatase AmsI from Erwinia amylovora.,Salomone-Stagni M, Musiani F, Benini S Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):903-910. Epub 2016, Nov 30. PMID:27917839[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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