6aun: Difference between revisions
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==calcium-independent phospholipase A2 beta== | |||
<StructureSection load='6aun' size='340' side='right' caption='[[6aun]], [[Resolution|resolution]] 3.95Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6aun]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AUN FirstGlance]. <br> | |||
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aun OCA], [http://pdbe.org/6aun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aun RCSB], [http://www.ebi.ac.uk/pdbsum/6aun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aun ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Calcium-independent phospholipase A2beta (iPLA2beta) regulates important physiological processes including inflammation, calcium homeostasis and apoptosis. It is genetically linked to neurodegenerative disorders including Parkinson's disease. Despite its known enzymatic activity, the mechanisms underlying iPLA2beta-induced pathologic phenotypes remain poorly understood. Here, we present a crystal structure of iPLA2beta that significantly revises existing mechanistic models. The catalytic domains form a tight dimer. They are surrounded by ankyrin repeat domains that adopt an outwardly flared orientation, poised to interact with membrane proteins. The closely integrated active sites are positioned for cooperative activation and internal transacylation. The structure and additional solution studies suggest that both catalytic domains can be bound and allosterically inhibited by a single calmodulin. These features suggest mechanisms of iPLA2beta cellular localization and activity regulation, providing a basis for inhibitor development. Furthermore, the structure provides a framework to investigate the role of neurodegenerative mutations and the function of iPLA2beta in the brain. | |||
The structure of iPLA2beta reveals dimeric active sites and suggests mechanisms of regulation and localization.,Malley KR, Koroleva O, Miller I, Sanishvili R, Jenkins CM, Gross RW, Korolev S Nat Commun. 2018 Feb 22;9(1):765. doi: 10.1038/s41467-018-03193-0. PMID:29472584<ref>PMID:29472584</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6aun" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Gross, R W]] | |||
[[Category: Jenkins, C M]] | |||
[[Category: Korolev, S]] | |||
[[Category: Koroleva, O]] | |||
[[Category: Malley, K]] | |||
[[Category: Miller, I]] | |||
[[Category: Sanishvili, R]] | |||
[[Category: Atp binding]] | |||
[[Category: Calcium-independent]] | |||
[[Category: Calmodulin binding]] | |||
[[Category: Hydrolase]] | |||
[[Category: Ipla2beta]] | |||
[[Category: Phospholipase]] | |||
[[Category: Pla2g6]] | |||
[[Category: Pnpla9]] | |||