2f8e: Difference between revisions
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|PDB= 2f8e |SIZE=350|CAPTION= <scene name='initialview01'>2f8e</scene>, resolution 2.90Å | |PDB= 2f8e |SIZE=350|CAPTION= <scene name='initialview01'>2f8e</scene>, resolution 2.90Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=U5P:URIDINE-5'-MONOPHOSPHATE'>U5P</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1u09|1U09]], [[1wne|1WNE]], [[2d7s|2D7S]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8e OCA], [http://www.ebi.ac.uk/pdbsum/2f8e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f8e RCSB]</span> | |||
}} | }} | ||
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[[Category: Perez-Luque, R.]] | [[Category: Perez-Luque, R.]] | ||
[[Category: Verdaguer, N.]] | [[Category: Verdaguer, N.]] | ||
[[Category: foot and mouth disease virus]] | [[Category: foot and mouth disease virus]] | ||
[[Category: protein primer]] | [[Category: protein primer]] | ||
[[Category: rna-dependent rna polymerase | [[Category: rna-dependent rna polymerase ,vpg protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:59:16 2008'' | ||
Revision as of 02:59, 31 March 2008
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| , resolution 2.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | RNA-directed RNA polymerase, with EC number 2.7.7.48 | ||||||
| Related: | 1U09, 1WNE, 2D7S
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Foot and Mouth Disease Virus RNA-dependent RNA polymerase in complex with uridylylated VPg protein
OverviewOverview
Picornavirus RNA replication is initiated by the covalent attachment of a UMP molecule to the hydroxyl group of a tyrosine in the terminal protein VPg. This reaction is carried out by the viral RNA-dependent RNA polymerase (3D). Here, we report the X-ray structure of two complexes between foot-and-mouth disease virus 3D, VPg1, the substrate UTP and divalent cations, in the absence and in the presence of an oligoadenylate of 10 residues. In both complexes, VPg fits the RNA binding cleft of the polymerase and projects the key residue Tyr3 into the active site of 3D. This is achieved by multiple interactions with residues of motif F and helix alpha8 of the fingers domain and helix alpha13 of the thumb domain of the polymerase. The complex obtained in the presence of the oligoadenylate showed the product of the VPg uridylylation (VPg-UMP). Two metal ions and the catalytic aspartic acids of the polymerase active site, together with the basic residues of motif F, have been identified as participating in the priming reaction.
About this StructureAbout this Structure
2F8E is a Single protein structure of sequence from Foot-and-mouth disease virus c. Full crystallographic information is available from OCA.
ReferenceReference
The structure of a protein primer-polymerase complex in the initiation of genome replication., Ferrer-Orta C, Arias A, Agudo R, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, EMBO J. 2006 Feb 22;25(4):880-8. Epub 2006 Feb 2. PMID:16456546
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