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==Atomic resolution structure of RNase A (data collection 5)== | |||
<StructureSection load='6eto' size='340' side='right' caption='[[6eto]], [[Resolution|resolution]] 1.02Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6eto]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ETO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ETO FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eto OCA], [http://pdbe.org/6eto PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eto RCSB], [http://www.ebi.ac.uk/pdbsum/6eto PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eto ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN]] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Despite their high relevance, the mechanisms of X-ray radiation damage on protein structure yet have to be completely established. Here, we used Raman microspectrophotometry to follow X-ray-induced chemical modifications on the structure of the model protein bovine pancreatic ribonuclease (RNase A). The combination of dose-dependent Raman spectra and ultrahigh resolution (eight structures solved using data collected between 0.85 and 1.17A resolution on the same single crystal) allowed direct observation of several radiation damage events, including covalent bond breakages and formation of radicals. Our results are relevant for analytical photodamage detection and provide implications for a detailed understanding of the mechanisms of photoproduct formation. | |||
Raman-markers of X-ray radiation damage of proteins.,Vergara A, Caterino M, Merlino A Int J Biol Macromol. 2018 Feb 2;111:1194-1205. doi:, 10.1016/j.ijbiomac.2018.01.135. PMID:29374529<ref>PMID:29374529</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6eto" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bos taurus]] | |||
[[Category: Pancreatic ribonuclease]] | |||
[[Category: Caterino, M]] | |||
[[Category: Merlino, A]] | |||
[[Category: Vergara, A]] | [[Category: Vergara, A]] | ||
[[Category: | [[Category: Atomic resolution]] | ||
[[Category: | [[Category: Hydrolase]] | ||
[[Category: Photodamage]] | |||
[[Category: Radiation damage]] | |||
[[Category: Raman microspectroscopy]] | |||
[[Category: Ribonuclease]] | |||
Revision as of 10:31, 22 February 2018
Atomic resolution structure of RNase A (data collection 5)Atomic resolution structure of RNase A (data collection 5)
Structural highlights
Function[RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.[1] Publication Abstract from PubMedDespite their high relevance, the mechanisms of X-ray radiation damage on protein structure yet have to be completely established. Here, we used Raman microspectrophotometry to follow X-ray-induced chemical modifications on the structure of the model protein bovine pancreatic ribonuclease (RNase A). The combination of dose-dependent Raman spectra and ultrahigh resolution (eight structures solved using data collected between 0.85 and 1.17A resolution on the same single crystal) allowed direct observation of several radiation damage events, including covalent bond breakages and formation of radicals. Our results are relevant for analytical photodamage detection and provide implications for a detailed understanding of the mechanisms of photoproduct formation. Raman-markers of X-ray radiation damage of proteins.,Vergara A, Caterino M, Merlino A Int J Biol Macromol. 2018 Feb 2;111:1194-1205. doi:, 10.1016/j.ijbiomac.2018.01.135. PMID:29374529[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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