2eb1: Difference between revisions
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|PDB= 2eb1 |SIZE=350|CAPTION= <scene name='initialview01'>2eb1</scene>, resolution 2.00Å | |PDB= 2eb1 |SIZE=350|CAPTION= <scene name='initialview01'>2eb1</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eb1 OCA], [http://www.ebi.ac.uk/pdbsum/2eb1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2eb1 RCSB]</span> | |||
}} | }} | ||
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[[Category: Tanokura, M.]] | [[Category: Tanokura, M.]] | ||
[[Category: Zenno, S.]] | [[Category: Zenno, S.]] | ||
[[Category: endonuclease]] | [[Category: endonuclease]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
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[[Category: rna-binding]] | [[Category: rna-binding]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:46:19 2008'' | ||
Revision as of 02:46, 31 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | |||||||
| Activity: | Ribonuclease III, with EC number 3.1.26.3 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the C-Terminal RNase III Domain of Human Dicer
OverviewOverview
Human Dicer contains two RNase III domains (RNase IIIa and RNase IIIb) that are responsible for the production of short interfering RNAs and microRNAs. These small RNAs induce gene silencing known as RNA interference. Here, we report the crystal structure of the C-terminal RNase III domain (RNase IIIb) of human Dicer at 2.0 A resolution. The structure revealed that the RNase IIIb domain can form a tightly associated homodimer, which is similar to the dimers of the bacterial RNase III domains and the two RNase III domains of Giardia Dicer. Biochemical analysis showed that the RNase IIIb homodimer can cleave double-stranded RNAs (dsRNAs), and generate short dsRNAs with 2 nt 3' overhang, which is characteristic of RNase III products. The RNase IIIb domain contained two magnesium ions per monomer around the active site. The distance between two Mg-1 ions is approximately 20.6 A, almost identical with those observed in bacterial RNase III enzymes and Giardia Dicer, while the locations of two Mg-2 ions were not conserved at all. We presume that Mg-1 ions act as catalysts for dsRNA cleavage, while Mg-2 ions are involved in RNA binding.
About this StructureAbout this Structure
2EB1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Homodimeric structure and double-stranded RNA cleavage activity of the C-terminal RNase III domain of human dicer., Takeshita D, Zenno S, Lee WC, Nagata K, Saigo K, Tanokura M, J Mol Biol. 2007 Nov 16;374(1):106-20. Epub 2007 Sep 8. PMID:17920623
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