1usz: Difference between revisions

Revision as of 18:05, 5 November 2007

SEMET AFAE-3 ADHESIN FROM ESCHERICHIA COLI

OverviewOverview

Pathogenic Escherichia coli expressing Afa/Dr adhesins are able to cause, both urinary tract and diarrheal infections. The Afa/Dr adhesins confer, adherence to epithelial cells via interactions with the human complement, regulating protein, decay accelerating factor (DAF or CD55). Two of the, Afa/Dr adhesions, AfaE-III and DraE, differ from each other by only three, residues but are reported to have several different properties. One such, difference is disruption of the interaction between DraE and CD55 by, chloramphenicol, whereas binding of AfaE-III to CD55 is unaffected. Here, we present a crystal structure of a strand-swapped trimer of wild type, DraE. We also present a crystal structure of this trimer in complex with, chloramphenicol, as well as NMR data supporting the binding position of, chloramphenicol within the crystal. The crystal structure reveals the, precise atomic basis for the sensitivity of DraE-CD55 binding to, chloramphenicol and demonstrates that in contrast to other, chloramphenicol-protein complexes, drug binding is mediated via, recognition of the chlorine "tail" rather than via intercalation of the, benzene rings into a hydrophobic pocket.

About this StructureAbout this Structure

1USZ is a Single protein structure of sequence from Escherichia coli with SO4 and CL as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.

ReferenceReference

High resolution studies of the Afa/Dr adhesin DraE and its interaction with chloramphenicol., Pettigrew D, Anderson KL, Billington J, Cota E, Simpson P, Urvil P, Rabuzin F, Roversi P, Nowicki B, du Merle L, Le Bouguenec C, Matthews S, Lea SM, J Biol Chem. 2004 Nov 5;279(45):46851-7. Epub 2004 Aug 24. PMID:15331605

Page seeded by OCA on Mon Nov 5 17:10:52 2007

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

Revision as of 15:59, 5 November 2007 view source OCA (talk \| contribs) Bots, Bureaucrats, Administrators 3,175,590 edits No edit summary ← Older edit		Revision as of 18:05, 5 November 2007 view source OCA (talk \| contribs) Bots, Bureaucrats, Administrators 3,175,590 edits No edit summary Newer edit →
Line 38:		Line 38:
	[[Category: upec]]		[[Category: upec]]

	''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:04:43 2007''		''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:10:52 2007''