1k4y: Difference between revisions
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==Crystal Structure of Rabbit Liver Carboxylesterase in Complex with 4-piperidino-piperidine== | ==Crystal Structure of Rabbit Liver Carboxylesterase in Complex with 4-piperidino-piperidine== | ||
<StructureSection load='1k4y' size='340' side='right' caption='[[1k4y]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='1k4y' size='340' side='right' caption='[[1k4y]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4PN:4-PIPERIDINO-PIPERIDINE'>4PN</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4PN:4-PIPERIDINO-PIPERIDINE'>4PN</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k4y OCA], [http://pdbe.org/1k4y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k4y RCSB], [http://www.ebi.ac.uk/pdbsum/1k4y PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k4y OCA], [http://pdbe.org/1k4y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k4y RCSB], [http://www.ebi.ac.uk/pdbsum/1k4y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k4y ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/1k4y_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/1k4y_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1k4y" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1k4y" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 00:29, 25 January 2018
Crystal Structure of Rabbit Liver Carboxylesterase in Complex with 4-piperidino-piperidineCrystal Structure of Rabbit Liver Carboxylesterase in Complex with 4-piperidino-piperidine
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedMammalian carboxylesterases cleave the anticancer prodrug CPT-11 (Irinotecan) into SN-38, a potent topoisomerase I poison, and 4-piperidino-piperidine (4PP). We present the 2.5 A crystal structure of rabbit liver carboxylesterase (rCE), the most efficient enzyme known to activate CPT-11 in this manner, in complex with the leaving group 4PP. 4PP is observed bound adjacent to a high-mannose Asn-linked glycosylation site on the surface of rCE. This product-binding site is separated from the catalytic gorge by a thin wall of amino acid side chains, suggesting that 4PP may be released through this secondary product exit pore. The crystallographic observation of a leaving group bound on the surface of rCE supports the 'back door' product exit site proposed for the acetylcholinesterases. These results may facilitate the design of improved anticancer drugs or enzymes for use in viral-directed cancer cotherapies. Structural insights into CPT-11 activation by mammalian carboxylesterases.,Bencharit S, Morton CL, Howard-Williams EL, Danks MK, Potter PM, Redinbo MR Nat Struct Biol. 2002 May;9(5):337-42. PMID:11967565[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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