2dtw: Difference between revisions
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|PDB= 2dtw |SIZE=350|CAPTION= <scene name='initialview01'>2dtw</scene>, resolution 2.40Å | |PDB= 2dtw |SIZE=350|CAPTION= <scene name='initialview01'>2dtw</scene>, resolution 2.40Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=2GS:2-O-METHYL-ALPHA-D-GALACTOPYRANOSE'>2GS</scene>, <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=2GS:2-O-METHYL-ALPHA-D-GALACTOPYRANOSE'>2GS</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1wbl|1wbl]], [[2d3s|2d3s]], [[1wbf|1wbf]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dtw OCA], [http://www.ebi.ac.uk/pdbsum/2dtw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dtw RCSB]</span> | |||
}} | }} | ||
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[[Category: Surolia, A.]] | [[Category: Surolia, A.]] | ||
[[Category: Vijayan, M.]] | [[Category: Vijayan, M.]] | ||
[[Category: agglutinin]] | [[Category: agglutinin]] | ||
[[Category: glycosylated protein]] | [[Category: glycosylated protein]] | ||
[[Category: legume lectin]] | [[Category: legume lectin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:39:27 2008'' | ||
Revision as of 02:39, 31 March 2008
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| , resolution 2.40Å | |||||||
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| Ligands: | , , , , , , , | ||||||
| Related: | 1wbl, 2d3s, 1wbf
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of basic winged bean lectin in complex with 2Me-O-D-Galactose
OverviewOverview
The crystal structure of the complexes of basic winged-bean lectin with galactose, 2-methoxygalactose, N-acetylgalactosamine and methyl-alpha-N-acetylgalactosamine have been determined. Lectin-sugar interactions involve four hydrogen bonds and a stacking interaction in all of the complexes. In addition, an N-H...O hydrogen bond involving the hydroxyl group at C2 exists in the galactose and 2-methoxygalactose complexes. An additional hydrophobic interaction involving the methyl group in the latter leads to the higher affinity of the methyl derivative. In the lectin-N-acetylgalactosamine complex the N-H...O hydrogen bond is lost, but a compensatory hydrogen bond is formed involving the O atom of the acetamido group. In addition, the CH(3) moiety of the acetamido group is involved in hydrophobic interactions. Consequently, the 2-methyl and acetamido derivatives of galactose have nearly the same affinity for the lectin. The methyl group alpha-linked to the galactose takes part in additional hydrophobic interactions. Therefore, methyl-alpha-N-acetylgalactosamine has a higher affinity than N-acetylgalactosamine for the lectin. The structures of basic winged-bean lectin-sugar complexes provide a framework for examining the relative affinity of galactose and galactosamine for the lectins that bind to them. The complexes also lead to a structural explanation for the blood-group specificity of basic winged-bean lectin.
About this StructureAbout this Structure
2DTW is a Single protein structure of sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1319-24. Epub 2006, Oct 18. PMID:17057334
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