2ds6: Difference between revisions
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|PDB= 2ds6 |SIZE=350|CAPTION= <scene name='initialview01'>2ds6</scene>, resolution 2.0Å | |PDB= 2ds6 |SIZE=350|CAPTION= <scene name='initialview01'>2ds6</scene>, resolution 2.0Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2ds5|2DS5]], [[2ds7|2DS7]], [[2ds8|2DS8]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ds6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ds6 OCA], [http://www.ebi.ac.uk/pdbsum/2ds6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ds6 RCSB]</span> | |||
}} | }} | ||
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[[Category: Park, E Y.]] | [[Category: Park, E Y.]] | ||
[[Category: Song, H K.]] | [[Category: Song, H K.]] | ||
[[Category: substrate binding domain]] | [[Category: substrate binding domain]] | ||
[[Category: zinc finger domain]] | [[Category: zinc finger domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:38:43 2008'' | ||
Revision as of 02:38, 31 March 2008
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| , resolution 2.0Å | |||||||
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| Ligands: | |||||||
| Related: | 2DS5, 2DS7, 2DS8
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the ZBD in the tetragonal crystal form
OverviewOverview
The degradation of ssrA(AANDENYALAA)-tagged proteins in the bacterial cytosol is carried out by the ClpXP protease and is markedly stimulated by the SspB adaptor protein. It has previously been reported that the amino-terminal zinc-binding domain of ClpX (ZBD) is involved in complex formation with the SspB-tail (XB: ClpX-binding motif). In an effort to better understand the recognition of SspB by ClpX and the mechanism of delivery of ssrA-tagged substrates to ClpXP, we have determined the structures of ZBD alone at 1.5, 2.0, and 2.5 A resolution in each different crystal form and also in complex with XB peptide at 1.6 A resolution. The XB peptide forms an antiparallel beta-sheet with two beta-strands of ZBD, and the structure shows a 1:1 stoichiometric complex between ZBD and XB, suggesting that there are two independent SspB-tail-binding sites in ZBD. The high-resolution ZBD:XB complex structure, in combination with biochemical analyses, can account for key determinants in the recognition of the SspB-tail by ClpX and sheds light on the mechanism of delivery of target proteins to the prokaryotic degradation machine.
About this StructureAbout this Structure
2DS6 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of SspB-tail recognition by the zinc binding domain of ClpX., Park EY, Lee BG, Hong SB, Kim HW, Jeon H, Song HK, J Mol Biol. 2007 Mar 23;367(2):514-26. Epub 2007 Jan 9. PMID:17258768
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