2dp3: Difference between revisions
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|PDB= 2dp3 |SIZE=350|CAPTION= <scene name='initialview01'>2dp3</scene>, resolution 2.1Å | |PDB= 2dp3 |SIZE=350|CAPTION= <scene name='initialview01'>2dp3</scene>, resolution 2.1Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span> | ||
|GENE= gltim ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5741 Giardia intestinalis]) | |GENE= gltim ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5741 Giardia intestinalis]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dp3 OCA], [http://www.ebi.ac.uk/pdbsum/2dp3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dp3 RCSB]</span> | |||
}} | }} | ||
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[[Category: Lopez-Velazquez, G.]] | [[Category: Lopez-Velazquez, G.]] | ||
[[Category: Reyes-Vivas, H.]] | [[Category: Reyes-Vivas, H.]] | ||
[[Category: alpha/beta barrel]] | [[Category: alpha/beta barrel]] | ||
[[Category: enzyme]] | [[Category: enzyme]] | ||
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[[Category: triosephosphate isomerase]] | [[Category: triosephosphate isomerase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:37:29 2008'' | ||
Revision as of 02:37, 31 March 2008
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| , resolution 2.1Å | |||||||
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| Ligands: | |||||||
| Gene: | gltim (Giardia intestinalis) | ||||||
| Activity: | Triose-phosphate isomerase, with EC number 5.3.1.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a double mutant (C202A/A198V) of Triosephosphate isomerase from giardia lamblia
OverviewOverview
Triosephosphate isomerase from the mesophile Giardia lamblia (GlTIM) is the only known TIM with natural disulfide bridges. We previously found that oxidized and reduced thiol states of GlTIM are involved in the interconversion between native dimers and higher oligomeric species, and in the regulation of enzymatic activity. Here, we found that trophozoites and cysts have different oligomeric species of GlTIM and complexes of GlTIM with other proteins. Our data indicate that the internal milieu of G. lamblia is favorable for the formation of disulfide bonds. Enzyme mutants of the three most solvent exposed Cys of GlTIM (C202A, C222A, and C228A) were prepared to ascertain their contribution to oligomerization and activity. The data show that the establishment of a disulfide bridge between two C202 of two dimeric GlTIMs accounts for multimerization. In addition, we found that the establishment of an intramonomeric disulfide bond between C222 and C228 abolishes catalysis. Multimerization and inactivation are both reversed by reducing conditions. The 3D structure of the C202A GlTIM was solved at 2.1 A resolution, showing that the environment of the C202 is prone to hydrophobic interactions. Molecular dynamics of an in silico model of GlTIM when the intramonomeric disulfide bond is formed, showed that S216 is displaced 4.6 A from its original position, causing loss of hydrogen bonds with residues of the active-site loop. This suggests that this change perturb the conformational state that aligns the catalytic center with the substrate, inducing enzyme inactivation.
About this StructureAbout this Structure
2DP3 is a Single protein structure of sequence from Giardia intestinalis. Full crystallographic information is available from OCA.
ReferenceReference
Disulfide bridges in the mesophilic triosephosphate isomerase from Giardia lamblia are related to oligomerization and activity., Reyes-Vivas H, Diaz A, Peon J, Mendoza-Hernandez G, Hernandez-Alcantara G, De la Mora-De la Mora I, Enriquez-Flores S, Dominguez-Ramirez L, Lopez-Velazquez G, J Mol Biol. 2007 Jan 19;365(3):752-63. Epub 2006 Oct 21. PMID:17095008
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