6eu6: Difference between revisions
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==Sensor Amt Protein== | |||
<StructureSection load='6eu6' size='340' side='right' caption='[[6eu6]], [[Resolution|resolution]] 1.98Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6eu6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EU6 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZDM:NONYL+4-O-ALPHA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE'>ZDM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eu6 OCA], [http://pdbe.org/6eu6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eu6 RCSB], [http://www.ebi.ac.uk/pdbsum/6eu6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eu6 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report on the structure and function of an ammonium sensor/transducer from the anammox bacterium "Candidatus Kuenenia stuttgartiensis" that combines a membrane-integral ammonium transporter domain with a fused histidine kinase. It contains a high-affinity ammonium binding site not present in assimilatory Amt proteins. The levels of phosphorylated histidine in the kinase are coupled to the presence of ammonium, as conformational changes during signal recognition by the Amt module are transduced internally to modulate the kinase activity. The structural analysis of this ammonium sensor by X-ray crystallography and small-angle X-ray-scattering reveals a flexible, bipartite system that recruits a large uptake transporter as a sensory module and modulates its functionality to achieve a mechanistic coupling to a kinase domain in order to trigger downstream signaling events. | |||
Signaling ammonium across membranes through an ammonium sensor histidine kinase.,Pfluger T, Hernandez CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA Nat Commun. 2018 Jan 11;9(1):164. doi: 10.1038/s41467-017-02637-3. PMID:29323112<ref>PMID:29323112</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: Andrade, S | <div class="pdbe-citations 6eu6" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Andrade, S L.A]] | |||
[[Category: Hernandez, C]] | [[Category: Hernandez, C]] | ||
[[Category: Pflueger, T]] | [[Category: Pflueger, T]] | ||
[[Category: Ammonium transporter]] | |||
[[Category: Histidine kinase]] | |||
[[Category: Membrane protein]] | |||
[[Category: Signalling]] | |||
Revision as of 21:31, 24 January 2018
Sensor Amt ProteinSensor Amt Protein
Structural highlights
Publication Abstract from PubMedSensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report on the structure and function of an ammonium sensor/transducer from the anammox bacterium "Candidatus Kuenenia stuttgartiensis" that combines a membrane-integral ammonium transporter domain with a fused histidine kinase. It contains a high-affinity ammonium binding site not present in assimilatory Amt proteins. The levels of phosphorylated histidine in the kinase are coupled to the presence of ammonium, as conformational changes during signal recognition by the Amt module are transduced internally to modulate the kinase activity. The structural analysis of this ammonium sensor by X-ray crystallography and small-angle X-ray-scattering reveals a flexible, bipartite system that recruits a large uptake transporter as a sensory module and modulates its functionality to achieve a mechanistic coupling to a kinase domain in order to trigger downstream signaling events. Signaling ammonium across membranes through an ammonium sensor histidine kinase.,Pfluger T, Hernandez CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA Nat Commun. 2018 Jan 11;9(1):164. doi: 10.1038/s41467-017-02637-3. PMID:29323112[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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