6f4a: Difference between revisions
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The | ==Yeast mitochondrial RNA degradosome complex mtEXO== | ||
<StructureSection load='6f4a' size='340' side='right' caption='[[6f4a]], [[Resolution|resolution]] 3.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6f4a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F4A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F4A FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6f3h|6f3h]], [[3rc3|3rc3]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f4a OCA], [http://pdbe.org/6f4a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f4a RCSB], [http://www.ebi.ac.uk/pdbsum/6f4a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f4a ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nuclease and helicase activities play pivotal roles in various aspects of RNA processing and degradation. These two activities are often present in multi-subunit complexes from nucleic acid metabolism. In the mitochondrial exoribonuclease complex (mtEXO) both enzymatic activities are tightly coupled making it an excellent minimal system to study helicase-exoribonuclease coordination. mtEXO is composed of Dss1 3'-to-5' exoribonuclease and Suv3 helicase. It is the master regulator of mitochondrial gene expression in yeast. Here, we present the structure of mtEXO and a description of its mechanism of action. The crystal structure of Dss1 reveals domains that are responsible for interactions with Suv3. Importantly, these interactions are compatible with the conformational changes of Suv3 domains during the helicase cycle. We demonstrate that mtEXO is an intimate complex which forms an RNA-binding channel spanning its entire structure, with Suv3 helicase feeding the 3' end of the RNA toward the active site of Dss1. | |||
Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components.,Razew M, Warkocki Z, Taube M, Kolondra A, Czarnocki-Cieciura M, Nowak E, Labedzka-Dmoch K, Kawinska A, Piatkowski J, Golik P, Kozak M, Dziembowski A, Nowotny M Nat Commun. 2018 Jan 8;9(1):97. doi: 10.1038/s41467-017-02570-5. PMID:29311576<ref>PMID:29311576</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6f4a" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli]] | |||
[[Category: Nowak, E]] | |||
[[Category: Nowotny, M]] | |||
[[Category: Razew, M]] | |||
[[Category: Helicase]] | |||
[[Category: Hydrolase]] | |||
[[Category: Mitochondria]] | |||
[[Category: Nuclease]] | |||
[[Category: Protein complex]] | |||
[[Category: Rna degradation]] | |||
Latest revision as of 10:11, 17 January 2018
Yeast mitochondrial RNA degradosome complex mtEXOYeast mitochondrial RNA degradosome complex mtEXO
Structural highlights
Publication Abstract from PubMedNuclease and helicase activities play pivotal roles in various aspects of RNA processing and degradation. These two activities are often present in multi-subunit complexes from nucleic acid metabolism. In the mitochondrial exoribonuclease complex (mtEXO) both enzymatic activities are tightly coupled making it an excellent minimal system to study helicase-exoribonuclease coordination. mtEXO is composed of Dss1 3'-to-5' exoribonuclease and Suv3 helicase. It is the master regulator of mitochondrial gene expression in yeast. Here, we present the structure of mtEXO and a description of its mechanism of action. The crystal structure of Dss1 reveals domains that are responsible for interactions with Suv3. Importantly, these interactions are compatible with the conformational changes of Suv3 domains during the helicase cycle. We demonstrate that mtEXO is an intimate complex which forms an RNA-binding channel spanning its entire structure, with Suv3 helicase feeding the 3' end of the RNA toward the active site of Dss1. Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components.,Razew M, Warkocki Z, Taube M, Kolondra A, Czarnocki-Cieciura M, Nowak E, Labedzka-Dmoch K, Kawinska A, Piatkowski J, Golik P, Kozak M, Dziembowski A, Nowotny M Nat Commun. 2018 Jan 8;9(1):97. doi: 10.1038/s41467-017-02570-5. PMID:29311576[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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