5y01: Difference between revisions
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==Acid-tolerant monomeric GFP, Gamillus, non-fluorescence (OFF) state== | |||
<StructureSection load='5y01' size='340' side='right' caption='[[5y01]], [[Resolution|resolution]] 2.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5y01]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y01 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y01 OCA], [http://pdbe.org/5y01 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y01 RCSB], [http://www.ebi.ac.uk/pdbsum/5y01 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y01 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The fluorescent protein (FP) color palette has greatly contributed to the visualization of molecular and cellular processes. However, most FPs lose fluorescence at a pH lower than their neutral pKa ( approximately 6), and this has hampered their application in acidic organelles (pH approximately 4.5-6.0). Currently, several cyan- and red-colored acid-tolerant FPs are available; however, there are few reports of acid-tolerant green FPs (GFPs) that are practically applicable to bioimaging. Here, we developed the acid-tolerant monomeric GFP "Gamillus" from the jellyfish Olindias formosa, with excellent brightness, maturation speed, and photostability. Results from X-ray crystallography and point mutagenesis suggest that across a broad pH range the acid tolerance is attributed to stabilization of deprotonation in the chromophore phenyl ring by forming a unique trans configuration. We demonstrate that Gamillus can serve as a molecular tag suitable for imaging in acidic organelles through autophagy-mediated molecular tracking to lysosomes. | |||
Acid-Tolerant Monomeric GFP from Olindias formosa.,Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T Cell Chem Biol. 2017 Dec 23. pii: S2451-9456(17)30432-4. doi:, 10.1016/j.chembiol.2017.12.005. PMID:29290624<ref>PMID:29290624</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5y01" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Matsuda, T]] | [[Category: Matsuda, T]] | ||
[[Category: Nagai, T]] | |||
[[Category: Nakashima, R]] | |||
[[Category: Sakurai, K]] | [[Category: Sakurai, K]] | ||
[[Category: | [[Category: Shinoda, H]] | ||
[[Category: | [[Category: Beta barrel]] | ||
[[Category: Cis-chromophore]] | |||
[[Category: Fluorescent protein]] | |||
[[Category: Gfp-like protein]] | |||
Revision as of 09:57, 17 January 2018
Acid-tolerant monomeric GFP, Gamillus, non-fluorescence (OFF) stateAcid-tolerant monomeric GFP, Gamillus, non-fluorescence (OFF) state
Structural highlights
Publication Abstract from PubMedThe fluorescent protein (FP) color palette has greatly contributed to the visualization of molecular and cellular processes. However, most FPs lose fluorescence at a pH lower than their neutral pKa ( approximately 6), and this has hampered their application in acidic organelles (pH approximately 4.5-6.0). Currently, several cyan- and red-colored acid-tolerant FPs are available; however, there are few reports of acid-tolerant green FPs (GFPs) that are practically applicable to bioimaging. Here, we developed the acid-tolerant monomeric GFP "Gamillus" from the jellyfish Olindias formosa, with excellent brightness, maturation speed, and photostability. Results from X-ray crystallography and point mutagenesis suggest that across a broad pH range the acid tolerance is attributed to stabilization of deprotonation in the chromophore phenyl ring by forming a unique trans configuration. We demonstrate that Gamillus can serve as a molecular tag suitable for imaging in acidic organelles through autophagy-mediated molecular tracking to lysosomes. Acid-Tolerant Monomeric GFP from Olindias formosa.,Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T Cell Chem Biol. 2017 Dec 23. pii: S2451-9456(17)30432-4. doi:, 10.1016/j.chembiol.2017.12.005. PMID:29290624[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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