6bu9: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
<StructureSection load='6bu9' size='340' side='right' caption='[[6bu9]], [[Resolution|resolution]] 6.80Å' scene=''> | <StructureSection load='6bu9' size='340' side='right' caption='[[6bu9]], [[Resolution|resolution]] 6.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6bu9]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BU9 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6bu9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BU9 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bu9 OCA], [http://pdbe.org/6bu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bu9 RCSB], [http://www.ebi.ac.uk/pdbsum/6bu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bu9 ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dcr-2, Dcr-2-RA, CG6493, Dmel_CG6493 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bu9 OCA], [http://pdbe.org/6bu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bu9 RCSB], [http://www.ebi.ac.uk/pdbsum/6bu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bu9 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Invertebrates rely on Dicer to cleave viral double-stranded RNA (dsRNA), and Drosophila Dicer-2 distinguishes dsRNA substrates by their termini. Blunt termini promote processive cleavage, while 3' overhanging termini are cleaved distributively. To understand this discrimination, we used cryo-electron microscopy to solve structures of Drosophila Dicer-2 alone and in complex with blunt dsRNA. While the Platform-PAZ domains have been considered the only Dicer domains that bind dsRNA termini, unexpectedly, we found that the helicase domain is required for binding blunt, but not 3' overhanging, termini. We further showed that blunt dsRNA is locally unwound and threaded through the helicase domain in an ATP-dependent manner. Our studies reveal a previously unrecognized mechanism for optimizing antiviral defense and set the stage for discovery of helicase-dependent functions in other Dicers. | |||
Dicer uses distinct modules for recognizing dsRNA termini.,Sinha NK, Iwasa J, Shen PS, Bass BL Science. 2017 Dec 21. pii: science.aaq0921. doi: 10.1126/science.aaq0921. PMID:29269422<ref>PMID:29269422</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6bu9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Drome]] | |||
[[Category: Bass, B L]] | [[Category: Bass, B L]] | ||
[[Category: Shen, P S]] | [[Category: Shen, P S]] | ||
Revision as of 09:05, 3 January 2018
Drosophila Dicer-2 bound to blunt dsRNADrosophila Dicer-2 bound to blunt dsRNA
Structural highlights
Publication Abstract from PubMedInvertebrates rely on Dicer to cleave viral double-stranded RNA (dsRNA), and Drosophila Dicer-2 distinguishes dsRNA substrates by their termini. Blunt termini promote processive cleavage, while 3' overhanging termini are cleaved distributively. To understand this discrimination, we used cryo-electron microscopy to solve structures of Drosophila Dicer-2 alone and in complex with blunt dsRNA. While the Platform-PAZ domains have been considered the only Dicer domains that bind dsRNA termini, unexpectedly, we found that the helicase domain is required for binding blunt, but not 3' overhanging, termini. We further showed that blunt dsRNA is locally unwound and threaded through the helicase domain in an ATP-dependent manner. Our studies reveal a previously unrecognized mechanism for optimizing antiviral defense and set the stage for discovery of helicase-dependent functions in other Dicers. Dicer uses distinct modules for recognizing dsRNA termini.,Sinha NK, Iwasa J, Shen PS, Bass BL Science. 2017 Dec 21. pii: science.aaq0921. doi: 10.1126/science.aaq0921. PMID:29269422[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||