1scj: Difference between revisions

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CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX

OverviewOverview

We report here the crystallographic structure determination of an, autoprocessed (Ser221Cys)-subtilisin E-propeptide complex at 2.0 A, resolution. The subtilisin domain sequence has a single substitution, (Ser221Cys) which has been shown to block the maturation process prior to, degradation of the propeptide domain (77 residues) that acts as an, intramolecular chaperon. This mutation, however, did not prevent the, enzyme from cleaving its propeptide domain with a 60-80% efficiency. The, current determination is the first example of a subtilisin E-propeptide, complex which has been autoprocessed. A previous structure determination, of a BPN'-prosegment complex has been reported in which the subtilisin, domain was extensively mutated and a calcium binding loop was deleted., Further, in this earlier determination, the complex was formed by the, addition of separately expressed propeptide domain. The structure, determination reported here provides additional information about the, nature of the interaction between the subtilisin and propeptide domains in, this complex.

About this StructureAbout this Structure

1SCJ is a Protein complex structure of sequences from Bacillus subtilis with CA as ligand. Active as Subtilisin, with EC number 3.4.21.62 Structure known Active Site: AVE. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution., Jain SC, Shinde U, Li Y, Inouye M, Berman HM, J Mol Biol. 1998 Nov 20;284(1):137-44. PMID:9811547

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	[[Category: subtilisin e - propeptide]]		[[Category: subtilisin e - propeptide]]

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