5fjl: Difference between revisions
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<StructureSection load='5fjl' size='340' side='right' caption='[[5fjl]], [[Resolution|resolution]] 1.47Å' scene=''> | <StructureSection load='5fjl' size='340' side='right' caption='[[5fjl]], [[Resolution|resolution]] 1.47Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5fjl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FJL FirstGlance]. <br> | <table><tr><td colspan='2'>[[5fjl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Raptor_adenovirus_a Raptor adenovirus a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FJL FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fjl OCA], [http://pdbe.org/5fjl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fjl RCSB], [http://www.ebi.ac.uk/pdbsum/5fjl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fjl ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fjl OCA], [http://pdbe.org/5fjl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fjl RCSB], [http://www.ebi.ac.uk/pdbsum/5fjl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fjl ProSAT]</span></td></tr> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Raptor adenovirus a]] | |||
[[Category: Nguyen, T H]] | [[Category: Nguyen, T H]] | ||
[[Category: Raaij, M J.van]] | [[Category: Raaij, M J.van]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
Revision as of 10:22, 13 December 2017
Crystal structure of raptor adenovirus 1 fibre head, wild-type formCrystal structure of raptor adenovirus 1 fibre head, wild-type form
Structural highlights
Publication Abstract from PubMedBACKGROUND: Most adenoviruses recognize their host cells via an interaction of their fibre head domains with a primary receptor. The structural framework of adenovirus fibre heads is conserved between the different adenovirus genera for which crystal structures have been determined (Mastadenovirus, Aviadenovirus, Atadenovirus and Siadenovirus), but genus-specific differences have also been observed. The only known siadenovirus fibre head structure, that of turkey adenovirus 3 (TAdV-3), revealed a twisted beta-sandwich resembling the reovirus fibre head architecture more than that of other adenovirus fibre heads, plus a unique beta-hairpin embracing a neighbouring monomer. The TAdV-3 fibre head was shown to bind sialyllactose. METHODS: Raptor adenovirus 1 (RAdV-1) fibre head was expressed, crystallized and its structure was solved and refined at 1.5 A resolution. The structure could be solved by molecular replacement using the TAdV-3 fibre head structure as a search model, despite them sharing a sequence identity of only 19 %. Versions of both the RAdV-1 and TAdV-3 fibre heads with their beta-hairpin arm deleted were prepared and their stabilities were compared with the non-mutated proteins by a thermal unfolding assay. RESULTS: The structure of the RAdV-1 fibre head contains the same twisted ABCJ-GHID beta-sandwich and beta-hairpin arm as the TAdV-3 fibre head. However, while the predicted electro-potential surface charge of the TAdV-3 fibre head is mainly positive, the RAdV-1 fibre head shows positively and negatively charged patches and does not appear to bind sialyllactose. Deletion of the beta-hairpin arm does not affect the structure of the raptor adenovirus 1 fibre head and only affects the stability of the RAdV-1 and TAdV-3 fibre heads slightly. CONCLUSIONS: The high-resolution structure of RAdV-1 fibre head is the second known structure of a siadenovirus fibre head domain. The structure shows that the siadenovirus fibre head structure is conserved, but differences in the predicted surface charge suggest that RAdV-1 uses a different natural receptor for cell attachment than TAdV-3. Deletion of the beta-hairpin arm shows little impact on the structure and stability of the siadenovirus fibre heads. Crystal structure of raptor adenovirus 1 fibre head and role of the beta-hairpin in siadenovirus fibre head domains.,Nguyen TH, Ballmann MZ, Do HT, Truong HN, Benko M, Harrach B, van Raaij MJ Virol J. 2016 Jun 22;13:106. doi: 10.1186/s12985-016-0558-7. PMID:27334597[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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