2bug: Difference between revisions
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|PDB= 2bug |SIZE=350|CAPTION= <scene name='initialview01'>2bug</scene> | |PDB= 2bug |SIZE=350|CAPTION= <scene name='initialview01'>2bug</scene> | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=ACE:ACETYL GROUP'>ACE</scene> | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bug OCA], [http://www.ebi.ac.uk/pdbsum/2bug PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bug RCSB]</span> | |||
}} | }} | ||
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[[Category: Ladbury, J E.]] | [[Category: Ladbury, J E.]] | ||
[[Category: Williams, M A.]] | [[Category: Williams, M A.]] | ||
[[Category: hsp90 binding]] | [[Category: hsp90 binding]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
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[[Category: tetratricopeptide domain]] | [[Category: tetratricopeptide domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:11:55 2008'' | ||
Revision as of 02:11, 31 March 2008
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| Ligands: | |||||||
| Activity: | Phosphoprotein phosphatase, with EC number 3.1.3.16 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF THE TPR DOMAIN FROM PROTEIN PHOSPHATASE 5 IN COMPLEX WITH HSP90 DERIVED PEPTIDE
OverviewOverview
Protein phosphatase 5 (Ppp5) is one of several proteins that bind to the Hsp90 chaperone via a tetratricopeptide repeat (TPR) domain. We report the solution structure of a complex of the TPR domain of Ppp5 with the C-terminal pentapeptide of Hsp90. This structure has the "two-carboxylate clamp" mechanism of peptide binding first seen in the Hop-TPR domain complexes with Hsp90 and Hsp70 peptides. However, NMR data reveal that the Ppp5 clamp is highly dynamic, and that there are multiple modes of peptide binding and mobility throughout the complex. Although this interaction is of very high affinity, relatively few persistent contacts are found between the peptide and the Ppp5-TPR domain, thus explaining its promiscuity in binding both Hsp70 and Hsp90 in vivo. We consider the possible implications of this dynamic structure for the mechanism of relief of autoinhibition in Ppp5 and for the mechanisms of TPR-mediated recognition of Hsp90 by other proteins.
About this StructureAbout this Structure
2BUG is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Conformational diversity in the TPR domain-mediated interaction of protein phosphatase 5 with Hsp90., Cliff MJ, Harris R, Barford D, Ladbury JE, Williams MA, Structure. 2006 Mar;14(3):415-26. PMID:16531226
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