1qjd: Difference between revisions
No edit summary |
No edit summary |
||
| Line 27: | Line 27: | ||
[[Category: respiratory fumarate reductase]] | [[Category: respiratory fumarate reductase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:00:39 2007'' | ||
Revision as of 17:55, 5 November 2007
|
FLAVOCYTOCHROME C3 FROM SHEWANELLA FRIGIDIMARINA
OverviewOverview
The 1.8 A resolution crystal structure of the tetraheme flavocytochrome, c3, Fcc3, provides the first mechanistic insight into respiratory fumarate, reductases or succinate dehydrogenases. The multi-redox center, three-domain protein shows a 40 A long 'molecular wire' allowing rapid, conduction of electrons through a new type of cytochrome domain onto the, active site flavin, driving the reduction of fumarate to succinate. In, this structure a malate-like molecule is trapped in the enzyme active, site. The interactions between this molecule and the enzyme suggest a, clear mechanism for fumarate reduction in which the substrate is polarized, and twisted, facilitating hydride transfer from the reduced flavin and, subsequent proton transfer. The enzyme active site in the oxidized form is, completely buried at the interface between the flavin-binding and the, clamp domains. Movement of the cytochrome and clamp domains is postulated, to allow release of the product.
About this StructureAbout this Structure
1QJD is a Single protein structure of sequence from Shewanella frigidimarina with NA, HEC, FAD, TEO and GOL as ligands. Structure known Active Site: SBS. Full crystallographic information is available from OCA.
ReferenceReference
Structural and mechanistic mapping of a unique fumarate reductase., Taylor P, Pealing SL, Reid GA, Chapman SK, Walkinshaw MD, Nat Struct Biol. 1999 Dec;6(12):1108-12. PMID:10581550
Page seeded by OCA on Mon Nov 5 17:00:39 2007