IspG: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Joel L. Sussman, Alexander Berchansky

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+<StructureSection load='4g9p' size='340' side='right' caption='IspG containing 4Fe4S cluster complex with MEcPP, MES, K+ (purple) and Cl- (green) ions (PDB entry [[4g9p]])' scene=''>
-<StructureSection load='4g9p' size='340' side='right' caption='IspG containing 4Fe4S cluster complex with MEcPP (PDB entry [[4g9p]])' scene=''>
 == Function ==
-IspG is a 4Fe4S protein which serves as the penultimate enzyme of the non-mevalonate pathway for the biosynthesis os isoprenoid precursors like isopentenyl diphosphate and dimethylallyl <ref>PMID:20932974</ref>. It catalyzes the ring opening of 2C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) producing (E)-1-hydroxyl-2-methyl-but-2-enyl-4-diphosphate (HMBPP).
+'''IspG''' is a 4Fe4S protein which serves as the penultimate enzyme of the non-mevalonate pathway for the biosynthesis os isoprenoid precursors like isopentenyl diphosphate and dimethylallyl <ref>PMID:20932974</ref>. It catalyzes the ring opening of 2C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) producing (E)-1-hydroxyl-2-methyl-but-2-enyl-4-diphosphate (HMBPP).
-== Disease ==
 == Relevance ==
-The non-mevlonate enzymes are a target for tuberculostatic and antimalarial drugs.
+The non-mevlonate enzymes are a target for tuberculostatic and antimalarial drugs<ref>PMID:22586085</ref>.
 == Structural highlights ==
+The substrate MEcPP is located in the cavity between the TIM-barrel and the lid domain of IpsG.  It binds covalently to the 4Fe4S cluster and its PP moiety binds to 2 Arg sidechains<ref>PMID:22967895</ref>.
 </StructureSection>
 == 3D Structures of IspG ==