2bjw: Difference between revisions
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjw OCA], [http://www.ebi.ac.uk/pdbsum/2bjw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bjw RCSB]</span> | |||
}} | }} | ||
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[[Category: Wigneshweraraj, S.]] | [[Category: Wigneshweraraj, S.]] | ||
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
[[Category: aaa]] | [[Category: aaa,transcription activation]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: dna-binding]] | [[Category: dna-binding]] | ||
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[[Category: pspf]] | [[Category: pspf]] | ||
[[Category: sigma54 activator]] | [[Category: sigma54 activator]] | ||
[[Category: transcription regulation,]] | |||
[[Category: transcription regulation]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:29 2008'' | ||
Revision as of 02:07, 31 March 2008
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| , resolution 1.75Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PSPF AAA DOMAIN
OverviewOverview
Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.
About this StructureAbout this Structure
2BJW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Escherichia coli
- Single protein
- Beuron, F.
- Bordes, P.
- Buck, M.
- Keetch, C A.
- Niwa, H.
- Rappas, M.
- Robinson, C V.
- Schumacher, J.
- Wigneshweraraj, S.
- Zhang, X.
- Aaa,transcription activation
- Atp-binding
- Dna-binding
- Enhancer binding protein
- Gene regulation
- Pspf
- Sigma54 activator
- Transcription regulation,