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==Structure of the Hemagglutinin-neuraminidase from Human parainfluenza virus type III: complex with difluorosialic acid==
==Structure of the Hemagglutinin-neuraminidase from Human parainfluenza virus type III: complex with difluorosialic acid==
<StructureSection load='4wef' size='340' side='right' caption='[[4wef]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='4wef' size='340' side='right' caption='[[4wef]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4wef]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WEF FirstGlance]. <br>
<table><tr><td colspan='2'>[[4wef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hpiv-3 Hpiv-3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WEF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DF4:(3R,4R,5R,6R)-5-(ACETYLAMINO)-3-FLUORO-4-HYDROXY-6-[(1R,2R)-1,2,3-TRIHYDROXYPROPYL]-3,4,5,6-TETRAHYDROPYRANIUM-2-CARBOXYLATE'>DF4</scene>, <scene name='pdbligand=FSI:5-(ACETYLAMINO)-3,5-DIDEOXY-3-FLUORO-D-ERYTHRO-ALPHA-L-MANNO-NON-2-ULOPYRANOSONIC+ACID'>FSI</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SFJ:(2R,3R,4R,5R,6R)-5-(ACETYLAMINO)-2,3-DIFLUORO-4-HYDROXY-6-[(1R,2R)-1,2,3-TRIHYDROXYPROPYL]TETRAHYDRO-2H-PYRAN-2-CARBOXYLIC+ACID'>SFJ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DF4:(3R,4R,5R,6R)-5-(ACETYLAMINO)-3-FLUORO-4-HYDROXY-6-[(1R,2R)-1,2,3-TRIHYDROXYPROPYL]-3,4,5,6-TETRAHYDROPYRANIUM-2-CARBOXYLATE'>DF4</scene>, <scene name='pdbligand=FSI:5-(ACETYLAMINO)-3,5-DIDEOXY-3-FLUORO-D-ERYTHRO-ALPHA-L-MANNO-NON-2-ULOPYRANOSONIC+ACID'>FSI</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SFJ:(2R,3R,4R,5R,6R)-5-(ACETYLAMINO)-2,3-DIFLUORO-4-HYDROXY-6-[(1R,2R)-1,2,3-TRIHYDROXYPROPYL]TETRAHYDRO-2H-PYRAN-2-CARBOXYLIC+ACID'>SFJ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1v3c|1v3c]], [[4weg|4weg]]</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1v3c|1v3c]], [[4weg|4weg]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wef OCA], [http://pdbe.org/4wef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wef RCSB], [http://www.ebi.ac.uk/pdbsum/4wef PDBsum]</span></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11216 HPIV-3])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wef OCA], [http://pdbe.org/4wef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wef RCSB], [http://www.ebi.ac.uk/pdbsum/4wef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wef ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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</div>
</div>
<div class="pdbe-citations 4wef" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 4wef" style="background-color:#fffaf0;"></div>
==See Also==
*[[Hemagglutinin|Hemagglutinin]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Hpiv-3]]
[[Category: Barrett, S]]
[[Category: Barrett, S]]
[[Category: McKimm-Breschkin, J]]
[[Category: McKimm-Breschkin, J]]

Revision as of 19:57, 16 November 2017

Structure of the Hemagglutinin-neuraminidase from Human parainfluenza virus type III: complex with difluorosialic acidStructure of the Hemagglutinin-neuraminidase from Human parainfluenza virus type III: complex with difluorosialic acid

Structural highlights

4wef is a 2 chain structure with sequence from Hpiv-3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, , , , , , ,
Gene:HN (HPIV-3)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The human parainfluenza virus type 3 (hPIV3) hemagglutinin-neuraminidase (HN) has opposing functions of binding sialic acid receptors and cleaving them, facilitating virus release. The crystal structure of hPIV3 HN complexed with the substrate analogue difluorosialic acid (DFSA) revealed that catalysis by HN involves the formation of a covalently linked sialosyl-enzyme intermediate which was trapped along with a transition-state analogue resembling an oxocarbenium ion. This mechanism of enzyme catalysis was also confirmed in the crystal structure of the influenza N9 neuraminidase complexed with DFSA. Additionally, novel secondary receptor binding sites were identified in the hPIV3 HN-DFSA complex including one near the catalytic cavity which upon binding DFSA imposes subtle changes and may help the HN balance the opposing functions. Multiple receptor binding sites may increase avidity to facilitate cell binding and fusion promotion. The secondary receptor binding sites in the paramyxoviruses are so far unique to each virus type.

Catalytic mechanism and novel receptor binding sites of human parainfluenza virus type 3 hemagglutinin-neuraminidase (hPIV3 HN).,Streltsov VA, Pilling P, Barrett S, McKimm-Breschkin JL Antiviral Res. 2015 Sep 10. pii: S0166-3542(15)00209-0. doi:, 10.1016/j.antiviral.2015.08.014. PMID:26364554[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Streltsov VA, Pilling P, Barrett S, McKimm-Breschkin JL. Catalytic mechanism and novel receptor binding sites of human parainfluenza virus type 3 hemagglutinin-neuraminidase (hPIV3 HN). Antiviral Res. 2015 Sep 10. pii: S0166-3542(15)00209-0. doi:, 10.1016/j.antiviral.2015.08.014. PMID:26364554 doi:http://dx.doi.org/10.1016/j.antiviral.2015.08.014

4wef, resolution 2.50Å

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