2b7t: Difference between revisions
No edit summary |
No edit summary |
||
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Adarb1, Red1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | |GENE= Adarb1, Red1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2b7v|2B7V]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b7t OCA], [http://www.ebi.ac.uk/pdbsum/2b7t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b7t RCSB]</span> | |||
}} | }} | ||
| Line 30: | Line 33: | ||
[[Category: rna-binding protein]] | [[Category: rna-binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:02:37 2008'' | ||
Revision as of 02:02, 31 March 2008
| |||||||
| Gene: | Adarb1, Red1 (Rattus norvegicus) | ||||||
| Related: | 2B7V
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of ADAR2 dsRBM1
OverviewOverview
Adenosine deaminases that act on RNA (ADARs) site-selectively modify adenosines to inosines within RNA transcripts, thereby recoding genomic information. How ADARs select specific adenosine moieties for deamination is poorly understood. Here, we report NMR structures of the two double-stranded RNA binding motifs (dsRBMs) of rat ADAR2 and an NMR chemical shift perturbation study of the interaction of the two dsRBMs with a 71 nucleotide RNA encoding the R/G site of the GluR-B. We have identified the protein and the RNA surfaces involved in complex formation, allowing us to present an NMR-based model of the complex. We have found that dsRBM1 recognizes a conserved pentaloop, whereas dsRBM2 recognizes two bulged bases adjacent to the editing site, demonstrating RNA structure-dependent recognition by the ADAR2 dsRBMs. In vitro mutagenesis studies with both the protein and the RNA further support our structural findings.
About this StructureAbout this Structure
2B7T is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
ReferenceReference
Structure and specific RNA binding of ADAR2 double-stranded RNA binding motifs., Stefl R, Xu M, Skrisovska L, Emeson RB, Allain FH, Structure. 2006 Feb;14(2):345-55. PMID:16472753
Page seeded by OCA on Mon Mar 31 02:02:37 2008