5dom: Difference between revisions
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==Crystal structure, maturation and flocculating properties of a 2S albumin from Moringa oleifera seeds== | ==Crystal structure, maturation and flocculating properties of a 2S albumin from Moringa oleifera seeds== | ||
<StructureSection load='5dom' size='340' side='right' caption='[[5dom]], [[Resolution|resolution]] 1.69Å' scene=''> | <StructureSection load='5dom' size='340' side='right' caption='[[5dom]], [[Resolution|resolution]] 1.69Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5dom]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DOM FirstGlance]. <br> | <table><tr><td colspan='2'>[[5dom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Horseradish_tree Horseradish tree]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DOM FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dom OCA], [http://pdbe.org/5dom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dom RCSB], [http://www.ebi.ac.uk/pdbsum/5dom PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dom OCA], [http://pdbe.org/5dom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dom RCSB], [http://www.ebi.ac.uk/pdbsum/5dom PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dom ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Horseradish tree]] | |||
[[Category: Arni, R K]] | [[Category: Arni, R K]] | ||
[[Category: Murakami, M T]] | [[Category: Murakami, M T]] | ||
Revision as of 17:11, 16 November 2017
Crystal structure, maturation and flocculating properties of a 2S albumin from Moringa oleifera seedsCrystal structure, maturation and flocculating properties of a 2S albumin from Moringa oleifera seeds
Structural highlights
Publication Abstract from PubMed2S albumins, the seed storage proteins, are the primary sources of carbon and nitrogen and are involved in plant defense. The mature form of Moringa oleifera (M. oleifera), a chitin binding protein isoform 3-1 (mMo-CBP3-1) a thermostable antifungal, antibacterial, flocculating 2S albumin is widely used for the treatment of water and is potentially interesting for the development of both antifungal drugs and transgenic crops. The crystal structure of mMo-CBP3-1 determined at 1.7 A resolution demonstrated that it is comprised of two proteolytically processed alpha-helical chains, stabilized by four disulfide bridges that is stable, resistant to pH changes and has a melting temperature (TM) of approximately 98 degrees C. The surface arginines and the polyglutamine motif are the key structural factors for the observed flocculating, antibacterial and antifungal activities. This represents the first crystal structure of a 2S albumin and the model of the pro-protein indicates the structural changes that occur upon formation of mMo-CBP3-1 and determines the structural motif and charge distribution patterns for the diverse observed activities. Crystal structure of mature 2S albumin from Moringa oleifera seeds.,Ullah A, Mariutti RB, Masood R, Caruso IP, Gravatim Costa GH, Millena de Freita C, Santos CR, Zanphorlin LM, Rossini Mutton MJ, Murakami MT, Arni RK Biochem Biophys Res Commun. 2015 Dec 4;468(1-2):365-71. doi:, 10.1016/j.bbrc.2015.10.087. Epub 2015 Oct 23. PMID:26505799[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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